Aicar 50mg / vial Tseem ceeb txhim kho Aerobic Metabolic Capacity thiab Endurance

AICAR 50mg / lub raj mis qhia

AMPK activator|Adenosine analogue 50mg / vial

Lub npeTom ntej: 5-aminoimidazole-4-formamide ribonucleoside

English npeTom ntej: 5-Aminoimidazole-4-carboxamide ribonucleoside (AICAR)

CAS Nr.: 2627-69-2

Cov mis mos molecular: C₉H₁₅N₄O₅

Molecular hnyavYog ': 259.24 g/mol

Qhov tshwm sim: Dawb rau tawm -dawb lyophilized hmoov

Cov ntsiab lus: 50mg / lub raj mis

Cia: Txias ntawm -20 degree, tiv thaiv lub teeb, thiab khaws cia rau hauv qhov chaw qhuav.

Daim ntawv qhia kev siv thiab khaws cia

AICAR (5-aminoimidazolium-4-carboxamide ribonucleoside):

AICAR yog ib qho adenosine analog uas tuaj yeem phosphorylated intracellularly los ntawm adenosine kinase los tsim ZMP (5-aminoimidazole-4-carboxamide ribonucleotide). ZMP, raws li AMP analog, tuaj yeem qhib AMP-activated protein kinase (AMPK). AMPK yog lub luag haujlwm tseem ceeb ntawm cov cellular zog metabolism, qhib rau hauv lub zog tsis muaj zog, txhawb nqa cov piam thaj, fatty acid oxidation, thiab mitochondrial biosynthesis thaum inhibiting protein thiab lipid synthesis. Los ntawm kev ua raws li lub zog tsis muaj zog, AICAR muab cov cuab yeej tshuaj tshuaj tseem ceeb rau kev kawm AMPK txoj kev taw qhia, kev tswj hwm lub zog metabolism, thiab cov txheej txheem cuam tshuam txog kab mob.

Vim nws qhov zoo - txhais tau tias AMPK ua kom cov nyhuv, AICAR tau siv dav hauv kev tshawb fawb kab mob metabolic, kev tawm dag zog lub cev, thiab tshawb nrhiav cov txheej txheem tiv thaiv kab mob plawv. Piv nrog rau lwm cov AMPK activators, nws cov txheej txheem ntawm kev ua haujlwm yog qhov tshwj xeeb, ua kom AMPK los ntawm kev hloov pauv hauv cov qib AMP endogenous. Qhov no ua rau nws yog ib qho cuab yeej tseem ceeb thiab kev kwv yees hauv cov qauv tsiaj rau kev kawm txog lub zog sib npaug, txhim kho cov tshuaj insulin, thiab tiv thaiv ischemia- raug mob rov qab.

AICAR Daim Ntawv Thov:

Intracellular phosphorylation generates ZMP, uas ua rau lub AMPK signaling txoj kev, txhawb cov piam thaj uptake, fatty acid oxidation, thiab mitochondrial muaj nuj nqi thaum inhibiting anabolism. AICAR yog tus tswj hwm cov metabolism raws li AMPK ua kom lub cev ua haujlwm, feem ntau yog siv los kawm txog lub zog metabolism, kev nkag siab ntawm insulin, thiab kev tawm dag zog.

AICAR tau siv dav hauv kev tshawb fawb txog kev mob metabolic, hom 2 mob ntshav qab zib, rog rog, kab mob plawv, thiab kev tawm dag zog lub cev. Nws zoo -txhais cov tshuaj pharmacological ntawm kev ua haujlwm muab qhov zoo tshaj - qauv kev tshawb fawb zoo rau kev soj ntsuam cov txheej txheem metabolic tshiab, kawm txog lub luag haujlwm ntawm AMPK hauv cov kab mob metabolic, thiab tshawb txog qhov cuam tshuam ntawm kev tawm dag zog ntawm lub zog metabolism.

Kev siv thiab kev khaws cia

1. Kev kho dua tshiab thiab kev npaj:

Cov kuab tshuaj pom zoo:

Nyiam cov kuab tshuaj: sterile dej rau kev txhaj tshuajlos yogsterile PBS tsis kam (pH 7.0-7.4)

Rau kev txhaj tshuaj sab hauv: dilute nrog sterile saline.

Lus ceeb toom: Tsis txhob siv cov kua qaub uas muaj zog, muaj zog alkalis, lossis cov tshuaj uas muaj cov kuab tshuaj organic.

Tus txheej txheem npaj:

Solvent npaj:

Cov kuab tshuaj tau lim los ntawm 0.22 μm lim membrane.

Qhov ntsuas kub hnyav tau rov qab los rau hauv chav sov (20-25 degree).

Reconstitution txheej txheem:

Ntxiv 1.0 mL ntawm cov kuab tshuaj pom zoo rau 50 mg vial.

Maj mam ncuav cov hmoov raws lub raj mis phab ntsa kom tsis txhob cuam tshuam ncaj qha rau cov hmoov.

Maj mam tig lub vial rau 3-5 feeb kom txog thaum yaj tag.

Qhov kawg concentration: 50 mg / mL (kwv yees 193 mM)

Dispensing thiab dilution:

Tam sim ntawd muab tso rau hauv ib zaug - siv koob tshuaj uas siv qis - nqus cryovials.

Dilute mus rau qhov yuav tsum tau ua hauj lwm concentration nrog sterile saline los yog PBS.

Clear labeling: Lub npe, concentration, npaj hnub, batch tooj

nruj me ntsis zam lub teeb thaum ua haujlwm.

Ua hauj lwm concentration/dosage reference:

Kev sim hauv vitro cell: Feem ntau siv cov concentration ntau yog 0.1 txog 1 mM

Tsiaj hauv vivo sim: Kev siv ntau npaum li cas yog 50 mus rau 500 mg / kg (intraperitoneal lossis intravenous txhaj).

Piv txwv ntawm kev npaj xam: Yog tias yuav tsum muaj 0.5 mM kev daws teeb meem, noj 2.6 μL ntawm cov tshuaj hauv Tshuag thiab ntxiv rau 1 mL ntawm kab lis kev cai nruab nrab.

Cov ntsiab lus tseem ceeb kom nco ntsoov:

Dissolve maj mam: Tsis txhob swirling lossis ua nruj ua tsiv; maj mam tig kom yaj.

Cov txheej txheem nruj aseptic: Kev ua haujlwm yog ua nyob rau hauv lub laminar flow hood, siv cov khoom siv tsis muaj menyuam.

Ua tiav kev tiv thaiv los ntawm lub teeb: Sensitive rau lub teeb, txhua kauj ruam yuav tsum tau ua nyob rau hauv lub teeb - tiv thaiv tej yam kev mob.

Npaj thiab siv tam sim ntawd: Kev ruaj ntseg yog txwv tom qab hloov kho, yog li nws raug pom zoo kom siv sai li sai tau.

Tsis txhob rov khov dua -thaw cycles: Zam ntau yam khov -thaw lub voj voog tom qab rov ntim dua.

2. Cia tej yam kev mob:

Unreconstituted lyophilized hmoov:

Ntev - lub sij hawm cia: Frozen ntawm -20 degree, txee lub neej 24 lub hlis

Kev cia zoo: -80℃(ntub khov), txee lub neej 36 lub hlis.

Kev tiv thaiv nruj ntawm lub teeb: Yuav tsum muab khaws cia rau hauv lub teeb qub- pov thawj aluminium foil hnab.

Kev tiv thaiv noo noo: Khaws qhuav, nrog ua -hauv desiccant.

Reconstituted daws:

tam sim ntawdtom qab kev kho dua tshiab rau kev ua haujlwm zoo.

Short - lub sij hawm cia: Txias ntawm 2-8℃deb ntawm lub teeb, tsis pub ntau tshaj 24 teev.

Repackage thiab khov: Khaws ntawm -20℃lossis -80℃deb ntawm lub teeb, tsis pub ntau tshaj 1 lub lis piam.

Rov qab khov-thaw cycles raug txwv nruj heev: qhov siab tshaj plaws ntawm ib qho khov -thaw voj voog.

Kev thauj mus los:

Txias- cov hmoov qhuav: thauj nrog dej khov qhuav (-78 degree)

Luv - lub sij hawm shipping: Thauj nrog cov pob khov nab kuab ntawm 2-8 degree, xyuas kom tus me nyuam tsis pub dhau 48 teev.

Ntim: Lub teeb- pov thawj aluminium foil hnab + lub tshuab nqus tsev foob + ua npuas ncauj insulated lub thawv

3. Kev ruaj ntseg thiab cov qauv ua tiav:

Tshuaj thiab lub cev stability:

Nws yog qhov ruaj khov nyob rau hauv aqueous daws nrog pH ntawm 7.0-7.4.

Tsis txhob sib cuag nrog cov kua qaub uas muaj zog, muaj zog alkalis, thiab oxidizing tus neeg sawv cev.

rhiab heev rau qhov kub thiab txias, lub sij hawm ntev raug rau chav tsev kub yuav tsum tau zam.

Kev kho ntawm bioactivity:

Kev xa tawm kom raug thiab khaws cia ntawm -80℃yog qhov tseem ceeb rau kev tswj xyuas kev ua haujlwm mus ntev.

Thaw thiab faib cov tshuaj maj mam rau ntawm cov dej khov ua ntej siv.

Xav taudab tsi, thov hu raupeb

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