Adipotide 2mg / vial Tshwj xeeb txo cov rog rog

Adipotide 2mg / lub raj mis qhia

Adipocyte-tig peptides|Dawb rog- tshwj xeeb pro-apoptotic peptides 2mg / vial

Hom npeTom ntej: Adipotide (FTPP)

English npe: CKGGRAKDC-GG-D(KLAKLAK)₂

Cov qauv sib txuas: Target peptide-linker-pro-apoptotic peptide (linear-cyclic complex qauv)

Molecular hnyav: kwv yees 2476.9 Da

Purity: Ntau dua lossis sib npaug li 95.0% (HPLC)

Cov ntsiab lus: 2mg / lub raj mis

Qhov tshwm sim: Dawb lyophilized hmoov

Cia: khov ntawm -20 degree, tiv thaiv lub teeb, thiab khaws cia rau hauv qhov chaw qhuav.

Daim ntawv qhia kev siv thiab cia

Adipotide (fat-targeting peptide):

Adipotide yog lub hom phiaj tshiab tshiab - kho mob chimeric peptide muaj li ntawm ob lub luag haujlwm ua haujlwm: N-terminal cyclic targeting peptide (CKGGRAKDC) uas tshwj xeeb khi rau annexin A2 receptor ntawm vascular endothelium ntawm cov ntaub so ntswg dawb; thiab C-terminal pro-apoptotic peptide (KLAKLAK)₂ uas cuam tshuam cov mitochondrial membrane, ua rau apoptosis. Qhov ob yog txuas los ntawm glycine-glycine linker. Tsis zoo li cov txheej txheem metabolic regulators, Adipotide precisely lub hom phiaj ntawm vascular kab mob ntawm cov ntaub so ntswg dawb, inducing adipocyte apoptosis thiab yog li xaiv txo cov dawb adipose ntaub so ntswg deposition. Nws qhov tshwj xeeb "cov phiaj xwm xa tawm-apoptosis induction" mechanism muab cov cuab yeej tshawb fawb hloov pauv rau kev kawm txog cov txheej txheem pathological thiab cov tswv yim kho tshiab ntawm kev rog rog, metabolic syndrome, thiab cov kab mob ntsig txog.

Vim nws lub hom phiaj tshwj xeeb ntawm cov ntaub so ntswg adipose thiab zoo -txhais pro-apoptotic mechanism, adipotide yog ib qho kev xaiv zoo rau cov kws tshawb fawb txog kev rog rog, kab mob metabolic, thiab vascular biology. Tsis zoo li cov tshuaj uas txo qhov hnyav los ntawm kev tswj kev noj qab haus huv lossis kev siv zog, nws lub tswv yim ntawm kev tshem tawm cov adipocytes ncaj qha muab qhov kev pom tshwj xeeb rau kev kawm txog adipose cov ntaub so ntswg biology, ntsuas cov kev kho mob hnyav tshiab, thiab tshawb nrhiav kev sib raug zoo ntawm cov ntaub so ntswg adipose thiab cov metabolism hauv lub cev. Nws cov rog sai thiab xaiv tau-txo cov teebmeem tshwm sim hauv cov qauv tsiaj ua rau nws muaj txiaj ntsig zoo rau cov cuab yeej tshawb fawb hauv kev sim cov qauv ntawm kev rog rog, cov rog hauv cheeb tsam, thiab cov kab mob metabolic.

Kev siv Adipotide:

Adipotide yog ib qho kev tshawb fawb tshiab peptide raws li kev ua haujlwm dual -kev ua haujlwm ntawm lub hom phiaj xa khoom thiab apoptosis induction. Nws khi tshwj xeeb rau cov hlab ntsha hauv cov ntaub so ntswg dawb ntawm nws lub hom phiaj, thiab tom qab internalization rau hauv cov hlwb, nws cov pro-apoptotic domain cuam tshuam cov mitochondrial membrane, inducing programmed cell tuag nyob rau hauv adipocytes, yog li xaiv txo cov ntaub so ntswg dawb. Nws yog feem ntau siv los tshawb txog cov txheej txheem pathological ntawm kev rog rog, vascular biology ntawm cov nqaij adipose, thiab cov tswv yim kho mob.

Adipotide yog dav siv hauv kev tshawb fawb txog kev rog rog, metabolic syndrome, lub regional adipose metabolism tsis meej, thiab adipose cov ntaub so ntswg angiogenesis. Nws zoo - txhais dual- lub luag haujlwm ntawm kev ua haujlwm muab cov qauv kev tshawb fawb tshwj xeeb rau kev soj ntsuam cov tswv yim kho tshiab uas tsom rau cov ntaub so ntswg adipose, kawm txog kev sib txawv ntawm cov ntaub so ntswg adipose dawb thiab xim av, thiab tshawb nrhiav lub luag haujlwm ntawm cov ntaub so ntswg adipose hauv cov metabolism hauv lub cev. Nws tseem yog ib qho tseem ceeb hauv kev ntsuam xyuas qhov ua tau zoo ntawm kev tiv thaiv - cov tshuaj rog rog thiab kawm txog cov txheej txheem ntawm kev rov ua dua tshiab thiab kev tuag ntawm adipocytes.

Kev siv thiab kev khaws cia

1. Kev kho dua tshiab thiab kev npaj:

Cov kuab tshuaj pom zoo:

Nyiam cov kuab tshuaj: sterile dej rau kev txhaj tshuajlos yogsterile phosphate tsis muaj pH 7.0-7.4

Kev ruaj ntseg optimization: Sterile PBS muaj 0.1% bovine serum albumin (BSA)(txo tsis yog{0}} tshwj xeeb adsorption).

Rau kev txhaj tshuaj sab hauv: dilute nrog sterile saline.

Lus ceeb toom: Vim nws cov qauv amphiphilic, tsis txhob siv cov kev daws teeb meem uas muaj cov kuab tshuaj organic lossis cov tshuaj detergents.

Tus txheej txheem npaj:

Solvent npaj:

Cov kuab tshuaj tau sterile lim los ntawm 0.22 μm lim membrane.

Qhov ntsuas kub hnyav tau rov qab los rau hauv chav sov (20-25 degree).

Reconstitution txheej txheem:

Ntxiv 1.0 mL ntawm cov kuab tshuaj pom zoo rau 2 mg vial.

Maj mam ncuav cov hmoov raws lub raj mis phab ntsa kom tsis txhob cuam tshuam ncaj qha rau cov hmoov.

Maj mam tig lub vial rau 3-5 feeb kom txog thaum yaj tag.

Qhov kawg concentration: 2 mg / mL (kwv yees li 0.81 mM)

Dispensing thiab dilution:

Tam sim ntawd muab tso rau hauv ib zaug - siv koob tshuaj (piv txwv li, 20-50 μL) siv cov tshuaj uas tsis tshua muaj adsorption cryovials.

Dilute rau kev ua haujlwm siab nrog PBS lossis physiological saline uas muaj 0.1% BSA.

Clear labeling: Lub npe, concentration, npaj hnub, batch tooj

nruj me ntsis zam lub teeb thaum ua haujlwm.

Ua hauj lwm concentration/dosage reference:

Kev sim hauv vitro cell: Feem ntau siv cov concentration ntau yog 0.1 μM txog 10 μM

Tsiaj hauv vivo sim: Qhov ntau npaum li cas yog 0.5 mus rau 5 mg / kg / hnub (intraperitoneal lossis subcutaneous txhaj), ua haujlwm tsis tu ncua rau ob peb hnub.

Piv txwv ntawm kev npaj xam: Yog tias xav tau 1 μM kev ua haujlwm, siv 1.23 μL ntawm cov tshuaj hauv Tshuag thiab ntxiv rau 1 mL ntawm kab lis kev cai nruab nrab.

Cov ntsiab lus tseem ceeb kom nco ntsoov:

Dissolve maj mam: Tsis txhob swirling lossis ua nruj ua tsiv kom tsis txhob muaj kev puas tsuaj rau nws cov qauv tsim.

Cov txheej txheem nruj aseptic: Kev ua haujlwm yog ua nyob rau hauv lub laminar flow hood, siv cov khoom siv tsis muaj menyuam.

Ua tiav kev tiv thaiv los ntawm lub teeb: Sensitive rau lub teeb, txhua kauj ruam yuav tsum tau ua nyob rau hauv lub teeb - tiv thaiv tej yam kev mob.

Npaj thiab siv tam sim ntawd: Kev ruaj ntseg yog txwv tom qab hloov kho, yog li nws raug pom zoo kom siv sai li sai tau.

Kom tsis txhob adsorption: siv cov tshuaj uas muaj 0.1% BSA los yog dilute nrog silanized qis-cov hlab adsorption kom txo tau cov adsorption ntawm phab ntsa raj thiab rab koob.

Concentration tswj: Tsis txhob npaj cov concentrations tsawg heev (<1 μM) for long-term storage to prevent degradation.

2. Cia tej yam kev mob:

Unreconstituted lyophilized hmoov:

Ntev - lub sij hawm cia: Frozen ntawm -20 degree, txee lub neej 18 lub hlis

Kev cia zoo: -80℃(ntub khov), txee lub neej 24 lub hlis.

Kev tiv thaiv nruj ntawm lub teeb: Yuav tsum muab khaws cia rau hauv lub teeb qub- pov thawj aluminium foil hnab.

Kev tiv thaiv noo noo: Khaws qhuav, nrog ua -hauv desiccant.

Reconstituted daws:

tam sim ntawdtom qab kev kho dua tshiab rau kev ua haujlwm zoo.

Short - lub sij hawm cia: Txias ntawm 2-8℃deb ntawm lub teeb, tsis pub ntau tshaj 12 teev.

Repackage thiab khov: Khaws ntawm -20℃lossis -80℃deb ntawm lub teeb tsis pub ntau tshaj 72 teev.

Rov qab khov-thaw cycles raug txwv nruj heev: qhov siab tshaj plaws ntawm ib qho khov -thaw voj voog.

Kev thauj mus los:

Txias- cov hmoov qhuav: Thauj nrog dej khov qhuav (-78 degree)

Ntim: Lub teeb- pov thawj aluminium foil hnab + lub tshuab nqus tsev foob + ua npuas ncauj insulated lub thawv

3. Kev ruaj ntseg thiab cov qauv ua tiav:

Tshuaj thiab lub cev stability:

Nws yog qhov ruaj khov hauv cov kev daws teeb meem tsis muaj pH ntawm 7.0-7.4.

Tsis txhob sib cuag nrog cov kua qaub uas muaj zog, muaj zog alkalis, oxidizing cov neeg ua haujlwm, thiab txo cov neeg ua haujlwm.

rhiab heev rau cov neeg kho tshuab kev nyuaj siab (xws li ua nruj ua tsiv los yog vortexing).

Kev kho ntawm bioactivity:

Kev xa tawm kom raug thiab khaws cia ntawm -80℃yog qhov tseem ceeb rau kev tswj xyuas kev ua haujlwm mus ntev.

Thaw thiab faib cov tshuaj maj mam rau ntawm cov dej khov ua ntej siv.

Xav taudab tsi, thov hu raupeb

Cim npe nrov: adipotide 2mg / vial tshwj xeeb txo cov roj deposition, Tuam Tshoj adipotide 2mg / vial tshwj xeeb txo cov roj deposition manufacturers, lwm tus neeg, Hoobkas