IGF-1 LR3 1mg/vial core introduction
Ntev - ua haujlwm insulin-zoo li kev loj hlob zoo-1 analog|Anabolic peptide 1mg / vial
Lub npe: Insulin-zoo li kev loj hlob zoo-1 ntev saw analog
English npe: Insulin-zoo li Growth Factor-1 Ntev R3 (IGF-1 LR3)
Ib ntus: Ib qho - saw polypeptide muaj li ntawm 83 amino acids, ib qho analog ntawm natural IGF-1, nrog 13-amino acid extension peptide ntxiv rau nws N-terminus, thiab glutamic acid ntawm txoj hauj lwm 3 hloov los ntawm arginine.
Molecular hnyav: kwv yees 9100 Da
Purity: Ntau dua lossis sib npaug li 97.0% (HPLC)
Cov ntsiab lus: 1 mg / fwj
Qhov tshwm sim: Dawb lyophilized hmoov
Cia: khov ntawm -20 degree, tiv thaiv lub teeb, thiab khaws cia rau hauv qhov chaw qhuav.
Daim ntawv qhia kev siv thiab cia
IGF-1 LR3 (ntev- ua haujlwm zoo li insulin-zoo li kev loj hlob-1):
IGF-1 LR3 yog qhov hloov kho analogue ntawm IGF ntuj-1. Los ntawm N- davhlau ya nyob twg txuas ntxiv thiab ib qho kev hloov pauv amino acid, nws txo qis kev sib koom ua ke rau insulin-zoo li kev loj hlob zoo- khi cov proteins, yog li txhim kho nws cov bioavailability thiab hauv vivo ib nrab - lub neej. Piv nrog rau ntuj IGF-1, nws pom tias muaj zog thiab muaj zog ntau dua cov dej num anabolic thiab mitotic. Raws li lub zog tswj kev loj hlob, nws ua lub luag haujlwm tseem ceeb hauv kev loj hlob ntawm tes, sib txawv, ciaj sia taus, thiab cov protein, carbohydrate, thiab lipid metabolism los ntawm kev ua kom cov insulin zoo li kev loj hlob factor-1 receptor. Tsis zoo li luv luv-acting ntuj peptides, nws qhov ua haujlwm ntev ua rau nws muaj txiaj ntsig zoo rau kev siv hauv cov leeg nqaij, kho cov ntaub so ntswg, kab lis kev cai ntawm tes, thiab kev tshawb fawb txog metabolic.
Vim nws txoj kev ua haujlwm zoo tshaj plaws ntawm anabolic thiab lub sijhawm ua haujlwm ntev ntev, IGF-1 LR3 yog qhov kev xaiv zoo tshaj plaws rau cov kws tshawb fawb hauv cov leeg nqaij, cov nqaij mos engineering, cell biology, thiab cov kev tshawb fawb metabolic. Piv rau endogenous IGF-1, nws txoj kev khi rau cov ntshav plasma-binding proteins raug txo, ua rau muaj ntau dua ntawm daim ntawv dawb huv thiab ua haujlwm ncaj qha thiab ua tau zoo. Qhov no muab cov cuab yeej muaj zog thiab ruaj khov rau kev kawm txog kev loj hlob ntawm cov leeg thiab pob txha, neuroprotection, cell culture expansion, thiab tsiaj cov qauv thiab hauv vitro thwmsim ntsig txog kev mob ntshav qab zib mellitus.
IGF-1 LR3 Daim Ntawv Thov:
Nws khi rau thiab qhib cov tshuaj insulin-zoo li kev loj hlob zoo-1 receptor nrog kev sib raug zoo siab, pib txoj hauv kev qis qis PI3K/Akt thiab MAPK, txhawb nqa kev loj hlob ntawm tes, sib txawv, thiab cov protein synthesis, thiab inhibiting apoptosis. IGF-1 LR3 yog ib qho muaj zog thiab ua haujlwm ntev anabolic raws li IGF-1 receptor signaling pathway, feem ntau yog siv rau hauv kev tshawb fawb txog kev loj hlob ntawm cov leeg, kho cov nqaij mos, kab lis kev cai ntawm tes, thiab kev tswj hwm cov txheej txheem metabolic.
IGF-1 LR3 yog siv dav hauv cov leeg nqaij pob txha hypertrophy thiab kev tsim dua tshiab, kev tshawb fawb osteoblast thiab chondrocyte, kev tiv thaiv cell neural thiab rov tsim dua tshiab, qia cell expansion thiab kab lis kev cai, thiab kev tshawb fawb txog ntshav qab zib mellitus. Nws zoo -txhais kev loj hlob- txhawb nqa thiab anabolistic mechanisms muab cov qauv kev tshawb fawb zoo rau kev ntsuam xyuas cov kev kho mob anabolic tshiab, tshawb nrhiav cov tswv yim kho cov ntaub so ntswg puas, ua kom cov kab mob ntawm tes ua kom zoo, thiab kawm txog kev loj hlob ntawm cov teeb liab transduction.
Kev siv thiab kev khaws cia
1. Kev kho dua tshiab thiab kev npaj:
Cov kuab tshuaj pom zoo:
Nyiam cov kuab tshuaj: Dilute acetic acid tov nrog pH 3.5-4.5 (piv txwv li, 0.1M acetic acid)
Kev ruaj ntseg optimization: Acetic acid tov ntawm pH 4.0 muaj 0.1% bovine serum albumin (BSA)(txo adsorption thiab aggregation).
Ua hauj lwm daws dilution: Dilute nrogsterile PBS lossis cell culture nruab nrab uas muaj 0.1% BSA.
Lus Cim Tseem Ceeb: Tsis txhob siv cov dej nruab nrab lossis alkaline tsis muaj menyuam lossis PBS rau kev hloov kho ncaj qha, vim qhov no tuaj yeem yooj yim ua rau cov protein sib sau ua ke, nag lossis daus, thiab inactivation. Tsis txhob tuav lub zog.
Tus txheej txheem npaj:
Solvent npaj:
Siv cov kua qaub acidic (0.1M acetic acid nrog pH ~ 4.0) uas tau ua kom tsis muaj menyuam lim los ntawm 0.22μm lim membrane.
Cia qhov ntsuas kub kom rov qab mus rau chav sov (20-25 degree).
Reconstitution txheej txheem:
Ntxiv 1.0 mL ntawm qhov pom zoo acidic hnyav rau 1 mg vial.
Maj mam ncuav cov hmoov raws lub raj mis phab ntsa kom tsis txhob cuam tshuam ncaj qha rau cov hmoov.
Muab lub vial horizontally thiabdov nws maj mam rov qab mus rau hauv koj txhais tes(tsis txhob vortex, co, los yog tshuab) kom txog rau thaum cov hmoov yog yaj tag thiab cov tshuaj ntshiab.
Qhov kawg concentration: 1 mg / mL (kwv yees li 0.11 mM) ntawm acidic stock solution.
Dispensing thiab dilution:
Tam sim ntawd muab tso rau hauv ib zaug - siv koob tshuaj (piv txwv li, 10-20 μL) siv cov khoom siv qis qis (xws li cov raj silanized).
Ua ntej siv, dilute cov kua qaub kua qaub tsawg kawg yog 1000 zaug rau kev ua haujlwm siab nrog PBS lossis kab lis kev cai nruab nrab uas muaj 0.1% BSA kom tsis txhob muaj pH.
Clear labeling: Lub npe, concentration, npaj hnub, batch tooj
nruj me ntsis zam lub teeb thaum ua haujlwm.
Ua hauj lwm concentration/dosage reference:
Kev sim hauv vitro cell: Feem ntau siv cov concentration ntau yog 1 ng / mL txog 100 ng / mL (kwv yees li 0.1 nM txog 11 nM).
Tsiaj hauv vivo sim: Feem ntau siv ntau npaum li cas yog 10 mus rau 200 ug / kg / hnub (subcutaneous txhaj).
Piv txwv ntawm kev npaj xam: Txhawm rau kom tau txais 10 ng / mL cov tshuaj ua haujlwm, ntxiv 1 μL ntawm cov tshuaj hauv Tshuag rau 100 mL ntawm kab lis kev cai nruab nrab uas muaj 0.1% BSA.
Cov ntsiab lus tseem ceeb kom nco ntsoov:
Acid soluble: Kev daws teeb meem pib yuav tsum tau ua nrog cov kua qaub acidic (pH 3.5-4.5), uas yog qhov tseem ceeb los tiv thaiv kev sib sau ua ke.
Txoj kev daws teeb meem: Dissolve los ntawm maj mam dov xwb. Ib qho kev tuav nruj heev tuaj yeem ua rau cov protein denaturation.
Kev ua haujlwm aseptic: Kev ua haujlwm aseptic yuav tsum tau ua nyob rau hauv lub laminar flow hood.
Saib xyuas qhov kev daws teeb meem: Cov tshuaj reconstituted yuav tsum ntshiab thiab tsis muaj xim. Txhua qhov kev daws teeb meem uas qhia tias muaj turbidity, flocculent teeb meem, los yog discoloration yuav tsum muab pov tseg.
Kev ntim khoom thiab ntim khoom: Acidic stock solutions can be aliquoted and stored at -20℃or -80℃. Diluted ua hauj lwm daws yuav tsum tau npaj thiab siv tam sim ntawd.
Tsis txhob adsorption: Siv cov khoom tsis muaj cov khoom thauj khoom (xws li 0.1% BSA) rau dilution thiab tuav kom txo cov adsorption rau phab ntsa raj.
2. Cia tej yam kev mob:
Unreconstituted lyophilized hmoov:
Ntev - lub sij hawm cia: Frozen ntawm -20 degree, txee lub neej 18 lub hlis
Kev cia zoo: -80℃(ntub khov), txee lub neej 24 lub hlis.
Kev tiv thaiv nruj ntawm lub teeb: Yuav tsum muab khaws cia rau hauv lub teeb qub- pov thawj aluminium foil hnab.
Kev tiv thaiv noo noo: Khaws qhuav, nrog ua -hauv desiccant.
Reconstituted solution (acidic stock solution):
Kev xa tawm tam sim: Dispense tam sim ntawd tom qab reconstitution.
Frozen cia: Tom qab rov ntim dua, khaws cia ntawm -20℃tsis pub ntau tshaj 1 lub hlis, lossis ntawm -80℃tsis pub ntau tshaj 3 lub hlis.
Rov qab khov-thaw cycles raug txwv nruj heev: Txhua lub raj xa dej tuaj yeem khov thiab thawed tsis pub ntau tshaj ib zaug.
Diluted ua haujlwm daws:
Npaj thiab siv tam sim ntawd: Cov tshuaj diluted nruab nrab ua hauj lwm yuav tsum tau siv nyob rau hauv 4 teev thiab yuav tsum tsis txhob muab khaws cia.
Kev thauj mus los:
Txias- cov hmoov qhuav: Yuav tsumyuav tsum thauj nyob rau hauv cov dej khov qhuav (-78 degree) tej yam kev mob.
Ntim: Lub teeb- pov thawj lub thawv + siab- lub zog insulated lub thawv + ntsuas kub ntsuas.
3. Kev ruaj ntseg thiab cov qauv ua tiav:
Tshuaj thiab lub cev stability:
Nws yog qhov ruaj khov hauv cov kua qaub aqueous (pH 3.5-4.5).
Nws yog qhov cuam tshuam rau kev sib sau ua ke, nag lossis daus, thiab deactivation nyob rau hauv nruab nrab lossis alkaline pH, siab ionic zog, siab kub, los yog mechanical shear force.
Cov ntsiab lus tseem ceeb rau kev tswj hwm bioactivity:
nruj me ntsis raws li lub hauv paus ntsiab lus ntawm "acidic reconstitution thiab nruab nrab siv"yog tus yuam sij rau kev tuav haujlwm.
Maj mam tuav thiab tsis txhob rov khov dua -thaw cycles yog qhov tseem ceeb.
Xav taudab tsi, thov hu raupeb
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Cim npe nrov: igf1-lr3 1mg/vial muaj zog thiab txhawb nqa kev loj hlob ntawm cov leeg nqaij, kho, thiab anabolism., Tuam Tshoj igf1-lr3 1mg/vial muaj zog thiab txhawb nqa kev loj hlob ntawm cov leeg, kho, thiab anabolism. manufacturers, lwm tus neeg, Hoobkas
