Kisspeptin 10mg / vial Optimize Reproductive Endocrine Function

Kisspeptin-10 10mg/vial core introduction

Gonadotropin{0}} tso tawm cov tshuaj hormones upstream regulatory peptide|Cov cim tseem ceeb peptide ntawm kev ua me nyuam neuroendocrine system 10mg / vial

Lub npe: Kisspeptin-10

Ib ntus: Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂

Molecular hnyav: Kwv yees li 1384.5 Da

Purity: Ntau dua lossis sib npaug li 98.5% (HPLC)

Qhov tshwm sim: Dawb lyophilized hmoov

Cia: Txias ntawm -20 degree, tiv thaiv lub teeb, thiab khaws cia rau hauv qhov chaw qhuav.

Daim ntawv qhia kev siv thiab khaws cia

Kisspeptin-10 neuropeptide:

Kisspeptin-10 yog ib qho tseem ceeb bioactive core decapeptide fragment ntawm tsev neeg kisspeptin. Raws li ib qho tseem ceeb ntawm kev tswj hwm ntawm hypothalamic gonadotropin - tso tawm cov tshuaj hormones neurons, nws ua lub luag haujlwm tseem ceeb hauv kev pib thiab kev tswj hwm ntawm kev ua me nyuam axis. Tsis zoo li cov tshuaj hormones ib txwm ua ncaj qha rau ntawm lub caj pas pituitary, nws tswj cov pulsatile tso tawm ntawm GnRH los ntawm tshwj xeeb ua rau GPR54 receptor, yog li tswj gonadotropin secretion. Nws qhov tshwj xeeb hypothalamic- theem kev tswj hwm txheej txheem ua rau nws hloov tsis tau nyob rau hauv txoj kev tshawb fawb txog kev pib hluas nkauj, kev tswj lub voj voog kev loj hlob, kev tawm tswv yim txog poj niam txiv neej, thiab cov qauv kab mob ntsig txog kev yug me nyuam.

Vim nws lub zog muaj zog GnRH- tso tawm stimulation thiab lub luag haujlwm tseem ceeb hauv lub cev xeeb tub, Kisspeptin-10 yog qhov kev xaiv zoo tshaj plaws rau ntau tus kws tshawb fawb hauv kev ua me nyuam endocrinology thiab neuroendocrinology. Nws tsis ua ncaj qha rau ntawm gonads, tab sis theej tawm nws cov teebmeem los ntawm txoj hauv kev zoo neuroendocrine, tsis txhob cuam tshuam ncaj qha ntawm cov kabmob peripheral. Nws cov yam ntxwv raws li "tus tswv hloov pauv" rau kev ua me nyuam ua rau nws yog ib qho tseem ceeb heev thiab cov cuab yeej sojntsuam tshwj xeeb hauv kev tshawb fawb ntawm hypogonadism, central precocious puberty, polycystic zes qe menyuam syndrome, thiab kev ua me nyuam muaj feem xyuam rau lub zog tshuav.

Kev siv Kisspeptin-10 neuropeptide:

Kisspeptin-10 tshwj xeeb txhawb kev tso tawm pulsatile ntawm GnRH los ntawm hypothalamus, yog li tswj kev tso tawm ntawm pituitary gonadotropins thiab pib thiab tswj kev ua me nyuam. Kisspeptin-10 yog ib qho tseem ceeb teeb liab peptide raws li neuroendocrine tswj mechanisms thiab yog ib tug tseem ceeb molecule txuas metabolic lub xeev thiab kev loj hlob muaj nuj nqi.

Kisspeptin-10 yog siv dav hauv kev tshawb fawb txog kev ua me nyuam physiology, neuroendocrinology, thiab kev cuam tshuam ntawm cov metabolism thiab kev yug me nyuam. Nws qhov tshwj xeeb ua kom cov chaw siab tshaj plaws ntawm kev ua me nyuam axis muab cov cuab yeej molecular tseem ceeb rau kev tshawb nrhiav cov etiology ntawm kev ua me nyuam tsis ua hauj lwm, tsim cov tswv yim tshiab kev loj hlob, thiab nkag siab txog cov txheej txheem uas cov khoom noj khoom haus thiab kev ntxhov siab cuam tshuam rau kev yug me nyuam. Nws kuj tseem muaj cov ntawv thov tseem ceeb hauv kev tshawb fawb cov qauv ntawm kev muaj menyuam tsis taus, kev loj hlob txawv txav, thiab cov kab mob endocrine cuam tshuam.

Txoj kev siv thiab khaws cia

1. Kev kho dua tshiab thiab kev npaj:

Cov kuab tshuaj pom zoo:

Nyiam cov kuab tshuaj: sterile dej rau kev txhaj tshuajlos yogsterile physiological saline (0.9% NaCl)

Kev ruaj ntseg optimization: Sterile dej muaj 0.1% bovine serum albumin (BSA)

Rau kev txhaj tshuaj / txhaj tshuaj: sterile artificial cerebrospinal kualos yognormal saline.

Tus txheej txheem npaj:

Solvent npaj:

Cov kuab tshuaj tau lim los ntawm 0.22 μm lim membrane.

Qhov ntsuas kub hnyav tau rov qab los rau hauv chav sov (20-25 degree).

Tsis txhob siv cov diluents uas muaj preservatives.

Reconstitution txheej txheem:

Ntxiv 1.0 mL ntawm cov kuab tshuaj pom zoo rau 10 mg vial.

Maj mam ncuav cov hmoov raws lub raj mis phab ntsa kom tsis txhob cuam tshuam ncaj qha rau cov hmoov.

Maj mam tig lub vial rau 3-5 feeb kom txog thaum yaj tag.

Qhov kawg concentration: 10 mg / mL (kwv yees li 7.2 mM)

Cais thiab khaws cia:

Tam sim ntawd muab tso rau hauv ib zaug - siv koob tshuaj 10-20 μL siv cov tshuaj qis qis qis.

Clear labeling: Lub npe, concentration, npaj hnub, batch tooj

nruj me ntsis zam lub teeb

Ua hauj lwm concentration/dosage reference:

Kev sim hauv vitro cell: Feem ntau siv cov concentration ntau yog 1 nM txog 1 μM

Tsiaj hauv vivo thwmsim (peripheral): Cov koob tshuaj ib txwm muaj yog 1 txog 10 nmol / kg (txoj kev txhaj tshuaj / subcutaneous).

Intraventricular txhaj rau tsiaj: Cov koob tshuaj ib txwm yog 1 txog 100 pmol / tsiaj txhu

Piv txwv ntawm kev npaj xam: Yog tias xav tau 1 μM kev daws teeb meem, siv 0.14 μL ntawm cov tshuaj hauv Tshuag thiab ntxiv rau 1 mL ntawm kab lis kev cai nruab nrab.

Cov ntsiab lus tseem ceeb kom nco ntsoov:

Dissolve maj mam: Tsis txhob swirling lossis ua nruj ua tsiv; yaj los ntawm maj mam tig.

Cov txheej txheem nruj aseptic: Kev ua haujlwm tau ua nyob rau hauv lub rooj ntev zaum huv si thiab siv cov khoom siv tsis muaj menyuam.

Kom tsis txhob muaj teeb pom kev zoo: Nws yog rhiab rau lub teeb, thiab tag nrho cov kauj ruam yuav tsum tau ua nyob rau hauv lub teeb - tiv thaiv tej yam kev mob.

Npaj thiab siv tam sim ntawd: Kev ruaj ntseg yog txwv tom qab hloov kho, yog li nws raug pom zoo kom siv sai li sai tau.

Kom tsis txhob adsorption: siv cov tshuaj uas muaj 0.1% BSA los yog dilute nrog silanized qis -cov hlab adsorption.

2. Cia tej yam kev mob:

Unreconstituted lyophilized hmoov:

Ntev - lub sij hawm cia: Khaws khov ntawm -20 degree, txee lub neej 24 lub hlis.

Kev cia zoo: Khaws khov ntawm -80 degree, txee lub neej 36 lub hlis.

Kev tiv thaiv nruj ntawm lub teeb: Yuav tsum muab khaws cia rau hauv lub teeb qub- pov thawj aluminium foil hnab.

Kev tiv thaiv noo noo: Khaws qhuav; muaj desiccant.

Reconstituted daws:

Siv tam sim ntawd: Rau kev ua haujlwm zoo tshaj plaws, siv tam sim tom qab hloov kho.

Short - lub sij hawm cia: Khaws ntawm 2-8℃deb ntawm lub teeb tsis pub ntau tshaj 8 teev.

Repackage thiab khov: Khaws ntawm -20℃lossis -80℃deb ntawm lub teeb tsis pub ntau tshaj 1 lub lis piam.

Rov qab khov-thaw cycles raug txwv nruj heev: qhov siab tshaj plaws ntawm ib qho khov nab kuab-thaw lub voj voog yuav ua rau txo qis zog.

Kev thauj mus los:

Txias- cov hmoov qhuav: thauj los ntawm cov dej khov qhuav (-78 degree).

Luv - lub sij hawm shipping: Thauj nrog cov pob khov nab kuab ntawm 2-8 degree, xyuas kom tus me nyuam tsis pub dhau 48 teev.

Ntim: Lub teeb- pov thawj aluminium foil hnab + lub tshuab nqus tsev foob + ua npuas ncauj insulated lub thawv.

3. Kev ruaj ntseg thiab cov qauv ua tiav:

Tshuaj thiab lub cev stability:

Nws yog qhov ruaj khov nyob rau hauv aqueous daws nrog pH ntawm 5.0-7.0.

Tsis txhob sib cuag nrog cov kua qaub uas muaj zog, muaj zog alkalis, oxidizing cov neeg ua haujlwm, thiab txo cov neeg ua haujlwm.

Nws yog rhiab heev rau cov neeg kho tshuab kev nyuaj siab (xws li kev quab yuam los yog vortex).

Kev kho ntawm bioactivity:

Kev xa tawm kom raug thiab khaws cia ntawm -80℃yog qhov tseem ceeb rau kev tswj xyuas kev ua haujlwm mus ntev.

Thaw cov tshuaj dispensed maj mam rau ntawm dej khov ua ntej siv.

Xav taudab tsi, thov hu raupeb

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