GDF-8 1mg/vial Tuam Tshoj Peptides Freeze-Powder qhuav

GDF-8 / Myostatin 1mg / lub raj mis Cov Lus Qhia

Pharmaceutical -qib recombinant protein|Tuam Tshoj - ua kom muaj kev sib txawv ntawm kev loj hlob 8 (GDF-8 / Myostatin )

Khoom npe: Recombinant Human Growth Differentiation Factor 8 (Myostatin)

UniProt ID: O14793

Specification: 1mg / lub raj mis (lyophilized hmoov)

Daim ntawv noj tshuaj: lyophilized protein raw khoom

Molecular hnyav: Kwv yees li 25 kDa (nyob rau hauv qhov txo qis)

Homodimer: Kwv yees li 50 kDa (tsis yog-txo cov xwm txheej)

Purity: Ntau dua lossis sib npaug li 95% (SDS-PAGE test)

Bioactivity: Txheeb xyuas los ntawm C2C12 cell proliferation inhibition assay

Cia kub: -20℃lossis -80℃rau kev cia ntev, 2-8℃rau lub sijhawm luv.

Kev thauj mus los: Kev thauj mus los qhuav

Solubility pom zoo: Sterile PBS (pH 7.2-7.4) lossis tsis muaj 0.1% BSA.

Cov teebmeem tseem ceeb

GDF-8 yog atus tswv cuab ntawm kev hloov pauv kev loj hlob - (TGF- ) superfamily. Raws liib tug tsis zoo regulator ntawm cov leeg loj hlob, nws muaj qhov tseem ceeb tshawb fawb tus nqi hauvkev txhim kho cov leeg nqaij, kev tshawb fawb nqaij atrophy, thiab txhim kho kev kho mob rau cov leeg nqaij.

✅Biologicaltshuab:

Cov leeg nqaij inhibition: inhibits myoblast proliferation thiab sib txawv los ntawm SMAD signaling pathway.

Receptor khi: Activates ActRIIB receptor, pib downstream signaling cascades.

Cov ntaub so ntswg tshwj xeeb: Feem ntau qhia thiab ua haujlwm hauv cov leeg pob txha.

✅Qualityqhov zoo ntawm Suav manufacturing:

Mammalian cell qhia: HEK293 los yog CHO cell qhia qhov system, xyuas kom zoo folding thiab glycosylation.

Affinity purification: Ni-NTA lossis antibody affinity chromatography, ob- txheej txheem purification.

Endotoxin tswj: <1.0 EU/μg, meeting the standards for injectable protein.

✅QualityCov qauv ntsuas:

SDS-PAGE purity: Ntau dua lossis sib npaug li 95% (Coomassie Brilliant Blue staining)

SEC-HPLC: Monomer cov ntsiab lus Ntau dua lossis sib npaug li 90%

Western Blot: Cov tshuaj tiv thaiv tshwj xeeb lees paub

Mass spectrometry tsom xam: MALDI-TOF lees paub qhov hnyav molecular

Kev tsom xam Peptide mapping: LC-MS/MS pov thawj ntawm cov amino acid sequences

Khoom nta thiab qhov zoo

1. Daim ntawv thov kev tshawb fawb tshawb fawb tus nqi:

Kev tshawb fawb txog nqaij mos biology: Kev tshawb fawb txog cov txheej txheem ntawm cov nqaij ntshiv kev loj hlob thiab rov tsim dua tshiab

Kab Mob Model Tsim: Cov cuab yeej tsim kom muaj Tus Qauv Muscular Atrophy

Tshuaj ntsuam xyuas platform: Kev tshuaj xyuas ntawm myostatin inhibitors

Kev tshawb nrhiav txoj hauv kev: TGF- /SMAD signaling pathway research

2. Technical parameters:

Kev nthuav qhia system: mammalian cell nthuav qhia, tsim nyog folding

Glycosylation: Kev hloov kho ntuj glycosylation nrog kev ua haujlwm raws li endogenous proteins.

Tsis system: 20 mM Tris -HCl, 150 mM NaCl, pH 8.0

Protein concentration: Ntau dua lossis sib npaug li 0.5 mg / ml ua ntej lyophilization

3. Qhov zoo ntawm kev tsim khoom hauv Suav teb:

Nqi kom zoo dua: Loj -scale ntau lawm txo cov nqi tshawb fawb thiab kev tsim kho

Advanced technology: Mature mammalian cell expression technology

Cov kev pabcuam Customization: Peb tuaj yeem muab cov khoom lag luam customized nrog cov ntawv sib txawv, mutants, thiab lwm yam.

Kev pab txhawb nqa: Pab pawg kws tshaj lij muab kev txhawb nqa daim ntawv thov.

Daim ntawv qhia kev siv thiab khaws cia

1. Cia tej yam kev mob:

Tsis qhib: tuaj yeem khaws cia rau 12 lub hlis ntawm -20 degree, thiab rau 24 lub hlis ntawm -80 degree.

Rov tsim dua: tuaj yeem khaws cia rau 7 hnub ntawm 2-8 degree, lossis rau 1 lub hlis ntawm -20 degree.

Tsis txhob rov khov dua -thaw cycles: Nws raug nquahu kom khaws cia rau hauv qhov me me thiab zam ntau tshaj 3 khov -thaw cycles.

Kev thauj mus los: Kev thauj mus los ntawm cov dej khov qhuav, kom ntseeg tau tias qhov kub thiab txias qis dua -60℃thoob plaws tag nrho cov lus.

2. Kev kho dua tshiab:

Kev npaj: Tshem tawm ntawm lub tub yees thiab cia kom sib npaug rau chav sov li 10 feeb.

Kev xaiv tsis zoo: Sterile PBS (pH 7.2-7.4) raug pom zoo.

Reconstitution concentration: Nws raug pom zoo kom rov ua dua rau 0.1-1.0 mg / ml.

Dissolving kauj ruam: Maj mam ntxiv cov tshuaj tsis haum thiab maj mam vortex sib tov.

Kev txiav txim siab concentration: Protein concentration tau txiav txim siab siv txoj kev BCA lossis A280 txoj kev.

3. Cov lus pom zoo rau kev siv hauv kev sim ntawm tes:

Ua haujlwm concentrationCov ntsiab lus pom zoo rau kev sim ntawm tes yog 1-100 ng / ml

Lub sijhawm kho: 24-72 teev rau kev kho mob ntawm tes

Kev tswj zoo: Nws raug pom zoo kom siv TGF- 1 raws li kev tswj hwm zoo.

Kev tswj tsis zoo: Teeb tsa cov ntshav qab zib- pub dawb kab lis kev cai nruab nrab tswj.

4. Cov lus pom zoo siv rau hauv kev sim tsiaj:

Kev noj tshuaj: Cov koob tshuaj pom zoo rau cov nas yog 1-10 mg / kg

Txoj kev tswj hwm: intraperitoneal txhaj los yog intramuscular txhaj

Lub sijhawm sim: Lub sij hawm muab tshuaj yog tsim raws li kev tshawb fawb lub hom phiaj.

Qauv sau: Sau cov ntaub so ntswg kuaj 24-72 teev tom qab ua tiav.

5. Ceev faj:

Cov txheej txheem aseptic: Tag nrho cov txheej txheem yuav tsum tau ua nyob rau hauv lub laminar flow hood.

Tsis txhob kis kab mob: Siv cov lus qhia tsis huv pipette thiab cov raj centrifuge.

Tsis txhob co: Tsis txhob tuav lub zog thaum lub sij hawm dissolution.

Npaj thiab siv tam sim ntawd: Nws raug nquahu kom npaj cov kev daws teeb meem ua haujlwm rau hnub ntawm kev sim.

Tshawb nrhiav thiab siv cov lus qhia

1. Cov kev tshawb fawb hauv paus:

Kev tshawb fawb txog cov txheej txheem molecular ntawm kev loj hlob ntawm cov leeg

Muscle stem cell biology tshawb fawb

Tshawb nrhiav cov txheej txheem tswj hwm ntawm cov leeg nqaij

2. Kev tshawb fawb txog kab mob:

Kev tshawb fawb ntawm pathogenesis ntawm muscular dystrophy

Kawm txog cachexia- cuam tshuam cov leeg nqaij

Hnub nyoog -kev tshawb fawb txog sarcopenia

3. Kev tshawb fawb txog tshuaj:

Kev tshuaj xyuas rau myostatin inhibitors

Antibody tshuaj txhim kho

Kev tshawb fawb ntawm Gene therapy

Vim li cas thiaj xaiv Suav - ua GDF-8 ?

Siabkev huv: SDS-PAGE purity Ntau dua lossis sib npaug li 95%, ua kom ntseeg tau ntawm cov txiaj ntsig kev sim.

Siabkev ua si: Qhia nyob rau hauv mammalian hlwb, ua kom zoo folding thiab glycosylation.

ruaj khovzoo: High batch-rau-batch sib xws ua kom muaj kev sim rov ua dua.

Cov kws tshaj lijTxhawb nqa: Muab cov ntaub ntawv ua tiav cov txheej txheem thiab cov ntawv qhia kev thov

Cov ntsiab lus

Cov khoom no yogsiab -purity recombinant human GDF-8/myostatin protein, manufactured sivadvanced mammalian cell qhia technology thiab ib tug nruj zoo tswj system nyob rau hauv Tuam Tshoj.Nws muablub siab -zoo, muaj zog tshawb nrhiav cov cuab yeejraunqaij biology thiab kev tshawb fawb txog kab mob. Nrogruaj khov thiab siv tau zoo, cov kws tshawb fawb tuaj yeem tau txaistxhim khu kev qha thiab rov tsim dua cov ntaub ntawv sim, nce qib kev tshawb fawb thiab kev tsim tshuaj rau cov leeg -cov kab mob.

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