HCG 5000iu / vial Regulating Reproductive Endocrine Function

Tib neeg Chorionic Gonadotropin (HCG) 5000 IU / vial: Core Taw Qhia

Tib neeg chorionic gonadotropin|Lub cev xeeb tub cov tshuaj hormones thiab LH mimic 5000 IU / vial

Lub npe: Tib neeg chorionic gonadotropin

Molecular qauv: Glycoprotein cov tshuaj hormones tsim los ntawm thiab subunits

Molecular hnyav: kwv yees 36.7 kDa

Purity: Ntau dua lossis sib npaug rau 95.0% (SEC-HPLC)

Cov haujlwm tshwj xeeb: Ntau dua lossis sib npaug li 5000 IU / vial

Qhov tshwm sim: Dawb lyophilized hmoov

Cia: Txias ntawm 2-8 degree, deb ntawm lub teeb thiab khaws cia rau hauv qhov chaw qhuav.

Daim ntawv qhia kev siv thiab cia

Tib neeg chorionic gonadotropin (HCG):

Tib neeg chorionic gonadotropin (HCG) yog ib qho glycoprotein hormone zoo li homologous rau luteinizing hormone (LH) hauv cov qauv. Nws ua raws li LH hauv nws qhov kev txiav txim ntawm cov qe ntshav interstitial hlwb, stimulating synthesis thiab secretion ntawm endogenous testosterone. Tsis zoo li cov tshuaj tiv thaiv kab mob androgen ncaj qha, HCG tswj lossis kho cov qog endocrine ib txwm ua haujlwm los ntawm kev ua kom muaj kev tawm tswv yim tsis zoo ntawm hypothalamus-pituitary-gonadal axis. Nws ua lub luag haujlwm tseem ceeb hauv kev kawm txog txiv neej gonadal kev ua haujlwm, kev tswj hwm spermatogenesis, thiab cov txheej txheem tawm tswv yim endocrine, thiab nquag siv los ua ib qho tseem ceeb rau kev soj ntsuam cov qog ntshav thiab tshawb fawb txog kev hloov tshuaj hormones.

Vim tias nws tuaj yeem ua raws li kev ua haujlwm lom neeg ntawm LH yam tsis tas yuav siv sijhawm ntev -cov kev tswj hwm ntawm cov tshuaj androgen exogenous, ntau tus kws tshawb fawb hauv kev tshawb fawb ntawm endocrinology thiab cov tshuaj tiv thaiv kev yug me nyuam xaiv siv HCG. Nws muaj txiaj ntsig zoo kom tsis txhob muaj cov qog ntshav tawm los ntawm exogenous testosterone, tswj cov qog endocrine thiab spermatogenic zog, thiab muab cov qauv kev tshawb fawb tseem ceeb rau kev kawm txog kev hloov tshuaj hormones, hypothalamic-pituitary-gonadal axis regulation, thiab pathophysiological mechanisms ntawm txiv neej infertility.

Kev siv tib neeg chorionic gonadotropin (HCG):

HCG khi rau LH receptors ntawm testicular interstitial hlwb, stimulating synthesis thiab secretion ntawm endogenous testosterone, txhawb spermatogenesis. HCG yog ib qho cuab yeej tseem ceeb hauv kev tshawb fawb txog kev xeeb tub endocrinology, siv los ntsuas kev ua haujlwm ntawm gonadal thiab cov txheej txheem tswj hwm.

HCG yog dav siv nyob rau hauv kev tshawb fawb ntawm kev ua me nyuam endocrinology, kev kho tus txiv neej infertility, thiab cov tshuaj hormone hloov kho cov tswv yim. Nws qhov zoo -txhais tau hais tias cov txheej txheem ntawm kev txiav txim ua rau nws yog ib qho tseem ceeb rau kev tshawb nrhiav kev ua haujlwm ntawm hypothalamus-pituitary-gonadal axis, ntsuam xyuas cov qog ntshav rau gonadotropins, thiab kawm txog kev tswj cov tshuaj hormonal tsis zoo. Nws kuj yog siv dav hauv kev tshawb fawb preclinical ntawm kev pabcuam kev ua menyuam yaus thiab kev ntsuas kev ua haujlwm gonadal.

Txoj kev siv thiab khaws cia

1. Kev kho dua tshiab thiab kev npaj:

Cov kuab tshuaj pom zoo:

Cov kuab tshuaj tshwj xeeb: Cov dej sterile nrog rau kev txhaj tshuaj

Lus ceeb toom: Tsis txhob siv cov kuab tshuaj, saline, lossis cov tshuaj tsis muaj tshuaj benzyl cawv lossis lwm yam tshuaj tua kab mob. Cov kuab tshuaj tsis raug tuaj yeem ua rau cov protein denaturation, sib sau ua ke, lossis txo qis hauv kev ua haujlwm.

Rau kev sim dilution: Tom qab rov kho dua nrog cov dej tsis huv rau kev txhaj tshuaj, nws tuaj yeem tsim nyog diluted nrogsterile physiological saline tsis muaj preservatives.

Tus txheej txheem npaj:

Solvent npaj:

Tshem tawm cov dej tsis huv nrog rau kev txhaj tshuaj los ntawm lub tub yees (2-8 degree) thiab cia nws sawv ntsug kom txog thaum nws ncav cuag chav sov.

Tag nrho cov txheej txheem tau ua raws li nruj raws li cov hauv paus ntsiab lus aseptic thiab nyob rau hauv lub txee biosafety.

Reconstitution txheej txheem:

Ntxiv 1.0-2.0 mL dej sterile rau txhaj rau 5000 IU vial.

Nrog rau lub koob kaum sab xis, maj mam txhaj cov kuab tshuaj raws lub raj mis phab ntsa kom tsis txhob cuam tshuam ncaj qha rau cov hmoov lyophilized thiab tsim cov pa npuas.

Maj mam tiglub vial horizontally nyob rau hauv xib-teg ntawm koj txhais tes, tsis muaj co los yog agitating, kom txog rau thaum cov hmoov yog yaj tag thiab cov tshuaj yog ntshiab.

Kev npaj siab: Piv txwv li, ntxiv 1.0 mL ntawm cov kuab tshuaj kom tau txais cov tshuaj hauv Tshuag ntawm 5000 IU / mL.

Dispensing thiab dilution:

Tom qab rov kho dua, nws yuav tsum tau muab faib rau hauv ib zaug - siv koob tshuaj, lossis diluted mus rau qhov xav tau qhov xav tau raws li qhov kev sim.

Dispense siv sterile, low-absorption centrifuge tubes.

Rau qis concentrations, dilute maj mam thiab maj mam nrog preservative - dawb saline tov.

Ua hauj lwm concentration/dosage reference:

Tsiaj hauv vivo sim: Kev siv ntau npaum li cas yog 50-1000 IU / kg. Cov koob tshuaj tshwj xeeb yog nyob ntawm hom tsiaj, kev sim qauv thiab kev siv ntau zaus (piv txwv li, ib zaug ib hnub lossis ob zaug hauv ib lub lis piam).

Kev sim hauv vitro cell: Feem ntau siv cov concentration ntau yog 1-100 IU / mL, uas yuav tsum tau ua kom zoo raws li cov kab ntawm tes thiab lub hom phiaj kev sim.

Lus ceeb toom: Cov koob tshuaj yuav tsum raug txiav txim kom raug los ntawm kev sim ua ntej. Kev noj ntau dhau tuaj yeem ua rau cov qib androgen siab tsis zoo thiab cuam tshuam txog kev phiv.

Cov ntsiab lus tseem ceeb kom nco ntsoov:

Solvent specification: Cov dej uas tsis muaj menyuam rau kev txhaj tshuaj yuav tsum tau siv.

Txoj kev daws teeb meem: Dissolve tsuas yog maj mam tig horizontally; Lub zog tuav yuav ua rau cov protein denaturation thiab inactivation.

Cov txheej txheem aseptic: Cov txheej txheem aseptic yuav tsum tau ua nyob rau hauv lub rooj zaum huv si lossis biosafety txee.

Saib xyuas qhov kev daws teeb meem: Cov tshuaj reconstituted yuav tsum ntshiab thiab tsis muaj xim. Yog hais tias turbidity, flocculent teeb meem, los yog discoloration tshwm sim, cov tshuaj yuav tsum tau muab pov tseg.

Npaj -rau- siv: Nws yuav tsum tau siv sai li sai tau tom qab hloov kho. Txawm hais tias tub yees, nws cov haujlwm yuav txo qis thaum lub sijhawm.

2. Cia tej yam kev mob:

Unreconstituted lyophilized hmoov:

Ntev - lub sij hawm cia: Txias ntawm 2-8 degrees. Txeelub neej yog 24 lub hlis (raws li cov khoom lag luam). Txias thiab lyophilizing hmoovyog txwv tsis pub.

Kev tiv thaiv nruj ntawm lub teeb: Yuav tsum muab khaws cia rau hauv lub teeb qub- pov thawj lub thawv sab nraud.

Kev tiv thaiv noo noo: Khaws qhuav; muaj desiccant.

Reconstituted daws:

Siv tam sim ntawd: Nws yuav tsum tau siv tam sim ntawd tom qab kev kho dua tshiab rau kev ua haujlwm zoo.

Short - lub sij hawm cia: Yog tias tsis siv tam sim ntawd, nws tuaj yeem tso rau hauv tub yees ntawm 2-8 degree,tab sis yuav tsum tau siv hauv 24 teev.

Kev khov yog txwv nruj heev: Reconstituted daws yuav tsum tsis txhob khov, vim tias khov yuav ua rau lawv cov qauv glycoprotein puas tsuaj, ua rau kev sib sau tsis tuaj yeem thiab tsis ua haujlwm.

Kev thauj mus los:

Kev thauj mus los txias: Yuav tsumyuav tsum tau thauj nyob rau hauv tub yees ntawm 2-8 degree, siv cov dej khov txaus thiab cov thawv insulated.

Ntim: Lub teeb- pov thawj lub thawv + siab- lub zog insulated lub thawv + ntsuas kub ntsuas.

3. Kev ruaj ntseg thiab cov qauv ua tiav:

ruaj khov:

Nws yog rhiab heev rau qhov kub thiab txias, mechanical shear force, pH tsis tsim nyog, thiab preservatives.

Nws yog qhov ruaj khov nyob rau hauv txoj cai hnyav, tab sis nws qhov ruaj khov txo qis tom qab kev sib cais.

Cov ntsiab lus tseem ceeb rau khaws cia cov khoom xyaw nquag:

Kev nruj me ntsis rau cov tub yees (tsis yog- khov) cov xwm txheej khaws cia yog qhov tseem ceeb rau kev tuav lub sijhawm ntev -.

Kev tuav maj mam yog qhov tseem ceeb rau kev tswj xyuas cov haujlwm tom qab rov tsim kho dua tshiab.

Xav taudab tsi, thov hu raupeb

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