AOD9604 2mg/vial Txhawb Kev Ua Rog Rog Thiab Ua kom lub cev muaj zog

AOD9604 2mg/bottle core introduction

Lipid metabolism regulating peptides|Kev loj hlob hormone fragment analogue 2mg / vial

Lub npeTIAB SA 9604

English npeTIAB SA 9604

Ib ntus: Tyr-hGly-Gln-hGly-Ser-Ser-Ser-Gln-hGly-Ser-Ser-Cys

Molecular hnyav: Kwv yees li 1158.3 Da

Purity: Ntau dua lossis sib npaug li 97.0% (HPLC)

Cov ntsiab lus: 2mg / lub raj mis

Qhov tshwm sim: Dawb lyophilized hmoov

Cia: khov ntawm -20 degree, tiv thaiv lub teeb, thiab khaws cia rau hauv qhov chaw qhuav.

Daim ntawv qhia kev siv thiab cia

AOD9604 (lipid metabolism regulating peptide):

AOD9604 yog ib qho kev hloov pauv peptide tau los ntawm C- ntu ntu ntawm tib neeg txoj kev loj hlob hormone (hGH) amino acid ib ntus, tsim los ua kom zoo li ib feem ntawm lipolytic kev ua haujlwm ntawm hGH thaum txo qis kev loj hlob - cuam tshuam txog kev pheej hmoo. Raws li kev tshawb fawb hluavtaws peptide, nws tau pom tias muaj peev xwm tswj cov khoom ntawm lipid metabolism hauv cov qauv kev sim, ntseeg tias yuav cuam tshuam lipid metabolism feem ntau los ntawm stimulating lipolysis thiab inhibiting lipogenesis. Tsis zoo li hGH tsis zoo, nws cov qauv tau ua kom zoo dua los cais nws cov lipid metabolism hauv kev tswj hwm kev ua haujlwm, yog li ua haujlwm tshwj xeeb hauv adipocyte biology, lub zog tshuav nyiaj li cas, thiab kev tshawb fawb txog kev mob metabolic.

Vim nws lub peev xwm los tswj kev tswj cov lipid metabolism, AOD9604 feem ntau xaiv los ntawm cov kws tshawb fawb hauv kev tshawb fawb metabolic, rog rog, thiab adipose biology. Piv nrog rau lwm yam dav -spectrum metabolic regulators, nws qhov tshwj xeeb tseem ceeb ntawm kev ua haujlwm ua rau nws ua cov qauv kev tshawb fawb reagent nyob rau hauv vitro thiab tsiaj qauv rau kev kawm dawb adipose ntaub so ntswg muaj nuj nqi, soj ntsuam cov tswv yim tshiab los txhawb lipolysis, thiab tshawb nrhiav lub luag hauj lwm ntawm lipid metabolism hauv kev rog thiab lwm yam kab mob.

AOD9604 Daim Ntawv Thov:

Raws li kev soj ntsuam peptide, nws yog siv rau hauv cov qauv kev sim tshwj xeeb los ntsuas nws qhov cuam tshuam rau adipocyte catabolism (lipolysis) thiab anabolism (lipogenesis). Nws lub hom phiaj tseem ceeb yog tshawb nrhiav cov txheej txheem tswj hwm cov ntaub so ntswg adipose, lub zog sib npaug, thiab rog rog -cov kab mob ntsig txog.

Nws tau raug siv hauv vitro kev tshawb fawb txog kev ua haujlwm ntawm adipocytes (xws li 3T3-L1 hlwb), tsiaj cov qauv ntawm kev noj zaub mov kom rog, thiab kev sim ntsuas cov tswv yim cuam tshuam rau cov kab mob metabolic. Nws cov qauv tshuaj muaj peev xwm muab cov cuab yeej tshawb fawb tseem ceeb rau kev kawm txog txoj hauv kev molecular ntawm lipid metabolism hauv kev tswj hwm thiab tshuaj xyuas cov peev txheej metabolic.

Kev siv thiab kev khaws cia

1. Kev kho dua tshiab thiab kev npaj:

Cov kuab tshuaj pom zoo:

Nyiam cov kuab tshuaj: Dilute acetic acid tov nrog pH 4.0-5.0 (piv txwv li, 0.1M acetic acid)

Kev ruaj ntseg optimization: Sterile acetic acid tov muaj 0.1% bovine serum albumin (BSA)

Ua hauj lwm daws dilution: Dilute rau nruab nrab pH nrogsterile PBS lossis cell culture nruab nrab uas muaj 0.1% BSA.

Lus ceeb toom: Do not use alkaline solutions (pH>7.5) los yog dej ntshiab kom yaj ncaj qha, vim qhov no yuav ua rau nag lossis daus.

Tus txheej txheem npaj:

Solvent npaj:

Siv cov kuab tshuaj acidic uas tau lim sterilely los ntawm 0.22 μm lim membrane.

Qhov ntsuas kub hnyav tau rov qab los rau hauv chav sov (20-25 degree).

Reconstitution txheej txheem:

Ntxiv 1.0 mL ntawm qhov pom zoo acidic hnyav rau 2 mg vial.

Ncuav maj mam raws lub raj mis phab ntsa kom tsis txhob cuam tshuam ncaj qha rau cov hmoov.

Maj mam tig lub vial rau 3-5 feeb kom txog thaum cov hmoov yog yaj tag thiab cov tshuaj ntshiab.

Qhov kawg concentration: 2 mg / mL (kwv yees li 1.73 mM) acidic stock solution.

Dispensing thiab dilution:

Tam sim ntawd muab tso rau hauv ib zaug - siv koob tshuaj 10-20 μL siv cov tshuaj qis qis.

Ua ntej siv, dilute cov kua qaub hauv Tshuag rau kev ua hauj lwm concentration nrog sterile PBS los yog kab lis kev cai nruab nrab uas muaj 0.1% BSA.

Daim ntawv qhia meej: npe, concentration, pH, hnub npaj, batch tooj.

Ua hauj lwm concentration/dosage reference:

Kev sim hauv vitro cell: Feem ntau siv cov concentration ntau yog 1 nM mus rau 1 μM, thiab kev sim ua ntej yuav tsum tau ua kom zoo dua qhov concentration raws li hom cell tshwj xeeb thiab lub hom phiaj kev sim.

Kev tshawb fawb tsiaj: Kev siv ntau npaum li cas yog 0.1 txog 1 mg / kg / hnub (intraperitoneal lossis subcutaneous txhaj).Nws raug pom zoolos txiav txim siab npaum li cas raws li cov qauv kev tshawb fawb tshwj xeeb thiab cov ntaub ntawv.

Piv txwv ntawm kev npaj xam: Yog tias yuav tsum muaj 1 μM kev daws teeb meem, siv li 0.58 μL ntawm cov tshuaj hauv Tshuag thiab ntxiv rau 1 mL ntawm kab lis kev cai nruab nrab (pH neutralization yuav tsum tau muab rau hauv tus account).

Cov ntsiab lus tseem ceeb kom nco ntsoov:

Acid solubility: Nws yuav tsum tau yaj nyob rau hauv ib qho chaw acidiclos xyuas kom meej solubility thiab stability.

Kov maj mam: Tsis txhob swirling lossis ua nruj ua tsiv; maj mam tig kom yaj.

Cov txheej txheem nruj aseptic: Kev ua haujlwm tau ua nyob rau hauv lub rooj ntev zaum huv si thiab siv cov khoom siv tsis muaj menyuam.

Kom tsis txhob muaj teeb pom kev zoo: Nws yog rhiab rau lub teeb, thiab tag nrho cov kauj ruam yuav tsum tau ua nyob rau hauv lub teeb - tiv thaiv tej yam kev mob.

Txhawm rau tiv thaiv adsorption: Siv cov tshuaj uas muaj 0.1% BSA thaum lub sij hawm dilution los txo cov adsorption ntawm phab ntsa raj.

2. Cia tej yam kev mob:

Unreconstituted lyophilized hmoov:

Ntev - lub sij hawm cia: khov ntawm -20 degree, txee lub neej 24 lub hlis.

Kev cia zoo: -80℃tob khov, txee lub neej 36 lub hlis.

Kev tiv thaiv nruj ntawm lub teeb: Yuav tsum muab khaws cia rau hauv lub teeb qub- pov thawj aluminium foil hnab.

Kev tiv thaiv noo noo: Khaws qhuav; muaj desiccant.

Reconstituted daws:

Kev xa tawm tam sim: Cov kua qaub kua qaub yuav tsum tau muab pov tseg tam sim tom qab rov tsim dua.

Frozen cia: Tom qab rov ntim dua, khaws cia ntawm -80℃deb ntawm lub teeb tsis pub ntau tshaj 1 lub hlis.

Kev daws teeb meem: Cov tshuaj diluted nruab nrab ua hauj lwm yuav tsum tau npaj thiab siv tam sim ntawd, thiab yuav tsum tau zam ntawm cia.

Rov qab khov-thaw cycles raug txwv nruj heev: Txhua lub raj xa dej tuaj yeem khov thiab thawed tsis pub ntau tshaj ib zaug.

Kev thauj mus los:

Txias- cov hmoov qhuav: Yuav tsumyuav tsum thauj nyob rau hauv cov dej khov qhuav (-78 degree) tej yam kev mob.

Ntim: Lub teeb- pov thawj lub thawv sab nraud + siab- lub zog insulated lub thawv + ntsuas kub ntsuas.

3. Kev ruaj ntseg thiab cov qauv ua tiav:

Tshuaj thiab lub cev stability:

Nws yog qhov ruaj khov hauv cov kua qaub aqueous (pH 4.0-5.0).

Nws solubility txo qis ntawm nruab nrab mus rau alkaline pH qib, thiab nws tuaj yeem tsim cov dej nag.

Tsis txhob sib cuag nrog cov kua qaub uas muaj zog, muaj zog alkalis, thiab oxidizing tus neeg sawv cev.

Kev kho ntawm bioactivity:

Ua tib zoo ua raws li lub hauv paus ntsiab lus ntawm "solving nyob rau hauv acidic daws thiab siv nyob rau hauv nruab nrab daws".

Maj mam tuav thiab tsis txhob rov khov dua -thaw cycles yog qhov tseem ceeb.

Xav taudab tsi, thov hu raupeb

Cim npe nrov: aod9604 2mg/vial txhawb kev rog rog thiab txhim kho lub cev muaj pes tsawg leeg, Tuam Tshoj aod9604 2mg / vial txhawb kev rog rog thiab txhim kho lub cev muaj pes tsawg leeg manufacturers, lwm tus neeg, Hoobkas