VIP 10mg / vial tswj lub paj hlwb, txhawb nqa vasodilation, thiab txhim kho kev ua pa

VIP Peptide 10mg / lub raj mis Cov Lus Qhia

Kev tshawb fawb-qib vasoactive plab hnyuv peptide|Ua nyob rau hauv Suav teb 28-peptide neuromodulator 10mg / lub raj mis

Lub npe: Vasoactive plab hnyuv peptide

Amino acid ua ntu zus: HSDAVFTDNYTRLRKQMAVKKYLNSILN-NH2

CAS Nr.: 40077-57-4

Cov mis mos molecular: C₁₄₇H₂₃₇N₄₃O₄₃S

Molecular hnyavYog ': 3325.8 g/mol

Purity: Ntau dua lossis sib npaug li 98.0% (HPLC kuaj pom)

Qhov tshwm sim: Dawb lyophilized hmoov

Cia: Khaws khov ntawm -20℃deb ntawm lub teeb; Tom qab thawing, refrigerate ntawm 2-8 degrees.

Core zoo thiab muaj nuj nqi

VIP (vasoactive plab hnyuv peptide) yog aneuropeptide muaj 28 amino acids, yogtsev neeg glucagon/secretin peptide. Raws liib qho tseem ceeb neurotransmitter thiab neuromodulator, VIPnce intracellular cAMP qib los ntawm kev ua kom VPAC1 thiab VPAC2 receptors,ua lub luag haujlwm dav dav hauv cov hlab ntsha, tiv thaiv kab mob, ua pa, plawv, thiab digestive systems. Cov khoom no yog ibCov cuab yeej tseem ceeb peptide rau kev kawm txog kev tswj hwm neuroimmunological, vasodilation, bronchodilation, kev ua haujlwm ntawm plab hnyuv, thiab tswj kev mob., thiab yogsiab -kawg reagent rau kev tshawb fawb ntawm ntau-system physiological thiab pathological mechanisms.

✅CoreMechanism ntawm Action:

Neural Regulation: Cov Neurotransmitters tseem ceeb hauv Central thiab Peripheral Nervous Systems

Vasodilation: Muaj zog so vascular du nqaij thiab ua kom cov nqaij mos ntshav txaus.

Kev tiv thaiv kab mob: Inhibits T cell proliferation thiab tswj macrophage muaj nuj nqi

Bronchiectasis: Relaxes airway smooth nqaij thiab txhim kho qhov cua

Kev ua haujlwm ntawm plab hnyuv: Txhawb txoj hnyuv plab thiab peristalsis.

Anti-cov nyhuv inflammatory: inhibits kev tso tawm ntawm pro-inflammatory cytokines

Neuroprotection: Txo excitotoxicity thiab tiv thaiv neurons

✅Qualityqhov zoo ntawm Suav manufacturers:

Khoom -theem synthesis txheej txheem:

Fmoc/tBu solid{0}}theem synthesis zoo

Microwave- pab synthesis txhim kho kev ua tau zoo

Synthesis yield Ntau dua lossis sib npaug li 75%, ua kom lub voj voog ntau lawm

Purification thiab kev tswj kom zoo:

Ob -theem purification: ion pauv chromatography ua ke nrog thim rov qab -theem HPLC

Purity tswj: Lub ntsiab ncov purity Ntau dua lossis sib npaug rau 98.0%, ib qho impurities Tsawg dua lossis sib npaug li 0.5%.

Kev txheeb xyuas ib ntus: Cov spectrometry loj tau lees paub qhov hnyav molecular, Edman degradation txheeb xyuas qhov sib lawv liag.

Kev soj ntsuam zoo:

Cov ntsiab lus Peptide: Ntau dua lossis sib npaug rau 95.0% (kev tshuaj xyuas amino acid)

Cov ntsiab lus acetate: Tsawg dua lossis sib npaug li 15.0%

Cov ntsiab lus noo noo: Tsawg dua lossis sib npaug li 5.0% (Karl Fischer txoj kev)

Endotoxin:<10 EU/mg

Sterility test: Ua tau raws li cov qauv tshuaj pharmacopoeia

Kev txheeb xyuas cov haujlwm:

Receptor binding assay: Txheeb xyuas affinity rau VPAC1 / VPAC2 receptors

Kev ua haujlwm ua haujlwm: cAMP kev sim ua kom muaj txiaj ntsig ua haujlwm lom neeg

Stability: Kawm txog kev daws teeb meem ruaj khov thiab khov -thaw stability

✅ khoomNta:

Siab bioactivity: Functionally validated siab bioactivity

Zoo stability: Lub khov- cov hmoov qhuav ua rau pom kev ruaj ntseg zoo heev.

Solubility zoo heev: yooj yim soluble hauv dej thiab tsis daws teeb meem.

Batch sib xws: Kev tswj xyuas nruj kom ntseeg tau qhov sib xws ntawm cov khoom siv.

Daim ntawv qhia kev siv thiab cia

1. Kev kho dua tshiab thiab kev npaj:

Cov kuab tshuaj pom zoo:

Nyiam cov kuab tshuajsterile 0.1% BSA / PBS kua (pH 7.4)

Kev sim ntawm tes: sterile DMSO (qhov kawg concentration<0.1%) or complete culture medium

Hauv vivo kev tswj hwm: sterile saline lossis saline uas muaj 0.1% BSA.

Standard configuration:

Ntxiv 1ml ntawm cov kuab tshuaj pom zoo thiab maj mam vortex kom yaj.

Ib qho kev daws teeb meem ntawm 10 mg / ml (kwv yees li 3 mM) tau txais.

Dilute mus rau kev ua haujlwm concentration nrog cov tshuaj tsis haum tsim nyog.

Tsis txhob ua nruj ua tsiv thiab rov tshuab dua.

Pom zoo ua haujlwm concentration:

Kev sim ntawm tes: 0.1-100 nM

Ex vivo lub cev: 1-100nM

Hauv vivo kev sim: 1-100 ug / kg

Receptor khi: 0.1-10nM

Cov kev ceev faj:

VIP tsis ruaj khov hauv kev daws teeb meem; Nws raug nquahu kom npaj nws tshiab txhua zaus koj siv nws.

Tsis txhob siv cov kua qaub uas muaj zog, muaj zog alkalis, lossis cov kev daws teeb meem uas muaj cov proteases.

Kev ua haujlwm yog nqa tawm ntawm cov dej khov kom txo qis degradation.

Khaws rau hauv qhov me me kom txo tau qhov khov -thaw cycles.

2. Cia tej yam kev mob:

Lyophilized hmoov:

Khaws ntawm -20℃hauv qhov chaw txias, qhuav kom deb ntawm lub teeb.

rau 24 lub hlisnyob rau hauv cov xwm txheej no

Tsis txhob rov khov dua -thaw cycles

Kev daws teeb meem:

Nws tuaj yeem khaws cia ntawm 4℃rau 24-48 teev.

Khaws ntawm -20℃rau 1 lub hlis (repackaged).

Nws raug nquahu kom ntxiv protease inhibitors.

Kev thauj mus los:

Kev thauj dej khov qhuav (-78 degree)

Lub sijhawm luv luv - lub sijhawm thauj cov pob khov nab kuab tuaj yeem ua tau.

Tsis txhob kub thiab tshav ntuj

3. Kev tshawb fawb thiab kev npaj ua ntawv thov:

Kev tshawb fawb hauv vitro:

Cell qauv:

Thawj kab lis kev cai neuron

astrocytes

microglia

macrophages

cov leeg nqaij du

Cov xwm txheej ua haujlwm:

Pretreatment: Stimulation yog siv 30 feeb tom qab kev kho mob VIP.

Co-kev kho mob: Ntxiv ib txhij nrog qhov ua kom muaj zog

Concentration gradient: Pom zoo 0.1, 1, 10, 100 nM

Kev ntsuas ntsuas:

CAMP theem: ELISA nrhiav

Inflammatory factors: TNF- , IL-1 , IL-6

Cell proliferation: CCK-8 los yog MTT

Kev kuaj pom Apoptosis: Annexin V / PI

Tsiaj hauv vivo kev tshawb fawb:

Kab mob qauv:

Tus qauv ntawm tus kab mob Parkinson

Tus qauv Alzheimer's disease

Asthma qauv

Tus qauv mob plab hnyuv kab mob

Sepsis qauv

Dosage regimen:

Intraperitoneal txhaj: 1-100 ug / kg, 1-2 zaug ib hnub.

Kev txhaj tshuaj: 1-50 ug / kg

Intraventricular txhaj: 1-10 ug / Tsiaj

Kev tswj hwm hauv zos: Xaiv raws li tus qauv

Kev kho lub voj voog:

Tus qauv mob: ib zaug lossis txuas ntxiv rau 3-7 hnub

Qauv: 2-4 lub lis piam

Kev ntsuas ntsuas:

Kev ntsuas tus cwj pwm

Histopathology

Inflammatory factor theem

Kev ntsuam xyuas kev ua haujlwm ntawm neurological

Mechanism ntawm kev kawm ua:

Receptor qhia: Western blot, immunohistochemistry

Kev taw qhia txoj kev: cAMP/PKA txoj kev ua kom

Gene expression: qPCR nrhiav pom cov noob muaj feem xyuam

Kev sib cuam tshuam ntawm cov protein: Co-IP, rub- nqis

4. Cov tswv yim tsim kev sim:

Kev tshawb nrhiav tshuaj: Teem 3-4 concentration gradients

Lub sijhawm cov ntsiab lus: Saib xyuas cov txiaj ntsig ntawm ntau lub sijhawm cov ntsiab lus

Kev tswj zoo: Ib tus paub VPAC receptor agonist tau siv.

Blockade kev sim: Siv cov receptor antagonists los xyuas qhov tseeb

Vim li cas thiaj xaiv VIP peptides ua hauv Suav teb?

Technical zoo:

28-peptide synthesis technology yog paub tab

Microwave synthesis txhim kho kev ua haujlwm

Advanced purification khoom

Ua kom zoo tswj qhov system

Kev lav zoo:

Purity Ntau dua lossis sib npaug li 98.0%

Ua kom tiav kev ua pov thawj

High batch sib xws

Muab tag nrho COA

Kev them nyiaj yug daim ntawv thov:

Cov lus qhia ntxaws ntxaws

Experimental scheme optimization

Kev pabcuam cov ntaub ntawv

Customized kho kom zoo nkauj kev pab cuam

Tus nqi -kev ua tau zoo:

Tus nqi tseem ceeb

Cov khoom ruaj khov thiab raws sijhawm

Ntau yam ntim specifications

Kev Txhawb Kev Txawj Ntse

Tshawb nrhiav thiab siv cov lus qhia

Kev tshawb nrhiav Neuroscience:

Cov kab mob neurodegenerative

Neuroinflammatory kev cai

Neuroprotective mechanisms

Kev kawm thiab kev nco muaj nuj nqi

Kev tshawb fawb txog kev tiv thaiv kab mob:

Cov kab mob autoimmune

Immune kam rau ua induction

Macrophage polarization

Cytokine cua daj cua dub

Ua pa system:

Bronchial hawb pob

Mob obstructive pulmonary kab mob

Pulmonary hypertension

Airway kho dua tshiab

digestive system:

Inflammatory plob tsis so tswj kab mob

Irritable plob tsis so tswj syndrome

Gastrointestinal motility disorders

pancreatitis

Cardiovascular system:

Kev tswj hwm vasodilatory

Kev tiv thaiv myocardial

Atherosclerosis

ntshav siab

Preclinical kev tshawb fawb cov ntaub ntawv siv

Kev ua haujlwm hauv vitro:

Ua kom VPAC receptors, EC50 kwv yees li 1 nM

Nce intracellular cAMP, EC50 kwv yees li 2 nM

Inhibits LPS -induced TNF- tso tawm, IC50 kwv yees li 10 nM

Sab hauv kho cov teebmeem:

Txhim kho MPTP-induced Parkinson cov tsos mob

Txo LPS{0}}induced neuroinflamation

Relieving OVA-cov tsos mob hawb pob

Kev txhim kho ntawm DSS-induced colitis

Pharmacokinetics:

Ib nrab luv luv -lub neej: kwv yees li 2-3 feeb

Kev faib tawm sai: feem ntau pom hauv lub ntsws, lub siab, thiab lub raum.

Metabolic sites: Feem ntau hauv lub ntsws thiab ob lub raum

Kev tshem tawm sai: degraded los ntawm peptidase

Kev ruaj ntseg:

Lub qhov rais kho yog qhov dav.

Tej yam tshwm sim tsis zoo: lub ntsej muag tawm, mob taub hau

Cov koob tshuaj ntau yuav ua rau cov ntshav qis

Tsis muaj kev cuam tshuam loj heev

Cov ntsiab lus

VIP, ibmultifunctional neuroimmunomodulatory peptide, tuav tus nqi tseem ceebhauv kev tshawb fawb txog ntau yam kab mob. Suav manufacturers, los ntawmadvanced synthesis technology, nruj tswj kev ua tau zoo, thiab kev ua tiav kev siv tau, muab cov kws tshawb fawbnrog siab - zoo, cov cuab yeej tshawb fawb muaj zog heev . Cov teebmeem lom neeg dav dav thiab zoo -txoj kev taw qhiantawm cov khoom no muaj kev txhawb zog rau kev tshawb fawb txog kev siv tshuab thiab kev tsim tshuaj hauv cov kab mob multisystemic.

Xav taudab tsi, thov hu raupeb

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