Follistatin 344 1mg/vial Muaj zog Inhibits Myostatin

Follicle-Stabilizing Txhaj 344 (Follistatin 344) 1mg/vial: Cov Nta Tseem Ceeb

Activin{0}}binding protein|Kev loj hlob zoo regulator 1mg / vial

Lub npe: Follicle-stimulating hormone 344

English npeTom ntej: Follistatin 344

Protein qauv: Recombinant protein, 344-amino acid follistatin isoform.

Molecular hnyav: Kwv yees li 38-42 kDa (tom qab glycosylation)

Purity: Ntau dua lossis sib npaug li 90% (SDS-PAGE/HPLC)

Cov ntsiab lus: 1 mg / fwj

Qhov tshwm sim: Dawb los yog tawm -dawb lyophilized hmoov

Cia: Txias ntawm -80 degree, tiv thaiv lub teeb, thiab khaws cia rau hauv qhov chaw qhuav.

Daim ntawv qhia kev siv thiab cia

Follistatin 344:

Follicle-staphylin 344 yog ib qho tshwm sim ntawm glycoprotein uas nws lub luag haujlwm tseem ceeb yog qhov siab -affinity binding protein thiab neutralizer rau kev hloov pauv txoj kev loj hlob- cov tswv cuab hauv tsev neeg xws li activin thiab myostatin. Los ntawm kev khi rau cov ligands, follicle-staphylin 344 ua haujlwm zoo thaiv lawv cov kev cuam tshuam nrog cov cell nto receptors, yog li tshem tawm cov teeb meem tsis zoo ntawm cov leeg nqaij. Hauv cov qauv kev sim, cov nyhuv inhibitory no txhawb nqa cov leeg nqaij pob txha ntawm cov leeg nqaij thiab kev sib txawv, thaum kawg ua rau cov leeg nqaij. Piv nrog rau kev txhaj tshuaj ncaj qha ntawm kev loj hlob, follicle-staphylin 344 siv nws cov teebmeem los ntawm kev hloov kho cov teeb liab endogenous network, muab cov cuab yeej tshwj xeeb rau kev kawm txog cov txheej txheem ntawm cov leeg nqaij hypertrophy, kho cov leeg nqaij atrophy, thiab tshawb nrhiav kev tswj cov metabolism.

Vim nws lub luag haujlwm tseem ceeb hauv txoj kev tswj hwm cov leeg nqaij, follistatin 344 tau siv dav hauv cov leeg nqaij biology, tshuaj kis las, cov kab mob neuromuscular (xws li cov leeg nqaij dystrophy), thiab kev tshawb fawb cachexia. Piv nrog rau cov inhibitors tsom rau ib leeg ligand (xws li tshuaj tiv thaiv -myosin antibodies), follistatin 344 muaj cov ligand binding spectrum, ua rau nws muaj kev tshawb fawb ntau dua rau kev kawm txog kev tswj hwm thoob ntiaj teb ntawm cov leeg nqaij loj hlob thiab rov tsim dua tshiab, tshuaj xyuas cov teebmeem synergistic, thiab tshawb nrhiav hauv nws cov qauv kev kho mob hauv lub cev. thiab mob cancer cachexia).

Kev thov ntawm follicle-stimulating hormone 344:

Raws li ib tug multifunctional endogenous binding protein, nws yog siv nyob rau hauv vitro thiab nyob rau hauv vivo qauv kom muaj zog khi rau thiab neutralize TGF- superfamily ligands xws li activin thiab myostatin, yog li kawm txog qhov dav ntawm kev thaiv txoj hauv kev no ntawm myoblast txoj hmoo kev txiav txim, skeletal leeg nqaij metabolic thiab kev ua haujlwm hauv tsev. Nws yog feem ntau siv los tshawb nrhiav cov tswv yim cuam tshuam rau cov kab mob nqaij atrophy, kho cov leeg nqaij thiab txhim kho tom qab raug mob, thiab cov leeg nqaij - rog metabolic kev sib cuam tshuam.

Nws yog ib txwm siv nyob rau hauv Duchenne cov leeg nqaij dystrophy nas qauv, cov leeg nqaij rov tsim dua tshiab, kev tshawb fawb txog hnub nyoog -txog sarcopenia, thiab kev tshawb fawb preclinical ntsuam xyuas cov tswv yim los txhawb cov leeg nqaij hypertrophy. Nws cov qauv tsim cov protein sib xyaw ua ke muab cov cuab yeej tshawb fawb tseem ceeb thiab txhim khu kev qha rau kev lees paub kho cov kev xav tau tsom rau TGF- superfamily signaling nyob rau hauv cov leeg nqaij thiab cov kab mob metabolic nyob rau hauv kev tswj xyuas nruj me ntsis.

Kev siv thiab kev khaws cia

1. Kev kho dua tshiab thiab kev npaj:

Cov kuab tshuaj pom zoo:

Nyiam cov kuab tshuaj: sterile dej rau kev txhaj tshuajlos yogsterile phosphate tsis nyob ntawm pH 6.0-7.4. Tsis txhob siv cov kuab tshuaj uas muaj pH siab heev.

Lub luag haujlwm tseem ceeb: Carrier protein yuav tsum tau ntxivrau reconstitution thiab tag nrho cov kev daws teeb meem.Sterile PBS muaj 0.1% -1% bovine serum albumin raug pom zookom txo qis qhov adsorption thiab nonspecific binding.

Ua hauj lwm daws dilution: Dilute nrogsterile PBS los yog cell kab lis kev cai nruab nrab uas muaj tib lub concentration ntawm carrier protein kom paub meej cov kev daws teeb meem zoo ib yam.

Ceeb toom tseem ceeb: Tsis txhob siv cov dej ntshiab los yog cov tshuaj tsis haum yam tsis muaj cov khoom thauj cov protein kom yaj ncaj qha. Tsis txhob vortex, co hnyav, los yog rov khov thiab thaw.

Tus txheej txheem npaj:

Solvent npaj:

Siv PBS (pH 7.2-7.4) los yog cov dej tsis huv rau kev txhaj tshuaj uas tau lim tsis huv los ntawm 0.22 μm lim membrane.

Ntxiv BSA uas tsis muaj protease thiab fatty acids rau cov kuab tshuaj kom ua tiav qhov kawg ntawm 0.1% -1%.

Cov kuab tshuaj raug pom zoo kom ua ua ntej-txias rau ntawm cov dej khov lossis npaj tshiab ntawm chav sov.

Reconstitution txheej txheem:

Ua cov txheej txheem hauv lub rooj zaum huv. Ntxiv 1.0 mL ntawm pre-txias cov cab kuj protein hnyav rau 1 mg vial.

Cia cov kuab tshuajntws los ntawm phab ntsa lub raj mis maj mam heevlos xyuas kom meej qhov ua tiav ntawm qhov khov - ncuav mog qab zib qhuav thiabzam kev cuam tshuam ncaj qha.

Muab lub vial rau ntawm dej khov los yog ntawm 4℃thiabcia nws yaj mus rau 15-30 feeb. Lub sijhawm no, koj tuaj yeem ua tauqeeb heev thiab maj mam qaij thiab tiglub vial ob peb zaug los pab dissolution.Tsis txhob vortex, co, lossis ua nruj ua tsiv.

Qhov kawg concentration: 1 mg / mL cov tshuaj hauv Tshuag.

Dispensing thiab gradient dilution:

Thaum yaj tag lawm, tam sim ntawd muab tso rau hauv ib zaug - siv koob tshuaj ntawm 5-20 μL siv cov khoom siv uas tsis tshua muaj adsorption silanized centrifuge raj rau dej khov.

Thaum diluting, siv PBS lossis kab lis kev cai nruab nrab uas muaj tib lub ntsiab lus ntawm cov cab kuj protein raugradient dilutionkom tsis txhob loj - nplai dilution hauv ib kauj ruam.

Daim ntawv lo ntshiab: Follistatin 344, concentration, carrier protein concentration, npaj hnub, batch tooj.

Ua haujlwm concentration siv:

Kev sim hauv vitro cell: Feem ntau siv cov concentration ntau yog 0.1 txog 5 ug / mL. Qhov tshwj xeeb concentration yuav tsum tau ua kom zoo dua los ntawm kev sim ua ntej raws li hom cell (xws li C2C12 myoblasts) thiab lub hom phiaj kev sim (kev loj hlob, kev sib txawv).

Hauv vivo tsiaj sim: Kev noj tshuaj yuav tsum nruj me ntsis raws li cov ntaub ntawv luam tawm ntawm tus qauv tsiaj tshwj xeeb. Feem ntau txoj kev ntawm kev tswj hwm yog intraperitoneal, subcutaneous, lossis intramuscular txhaj, nrog ntau npaum li cas feem ntau ntawm ug / kg txog mg / kg.Precise npaum li cas yuav tsum raug txiav txim raws li qhov kev sim ua ntej thiab cov kev cai ntawm tsiaj.

Piv txwv suav: Txhawm rau npaj cov tshuaj ua haujlwm 100 ng / mL, noj 1 μL ntawm cov tshuaj 1 mg / mL thiab ntxiv rau 1 mL ntawm nruab nrab uas muaj 0.1% BSA kom tau txais 1 ug / mL cov tshuaj nruab nrab. Tom qab ntawd noj 100 μL ntawm cov tshuaj nruab nrab thiab ntxiv rau 900 μL ntawm qhov nruab nrab.

Cov ntsiab lus tseem ceeb kom nco ntsoov:

Carrier proteins yog qhov tseem ceeb: Cov kev daws teeb meem uas tsis muaj cov cab kuj tuaj yeem ua rau poob cov protein ntau vim yog adsorption thiab tuaj yeem ua rau sib sau ua ke.

Siab mos heev: Txhua qhov kev ua haujlwm yuav tsum zam kom tsis txhob tsim cov npuas, ua npuas ncauj, los yog shear quab yuamlos tiv thaiv cov protein conformational hloov thiab inactivation.

Ntuj tawg: Cia sij hawm txaus rau cov protein kom yaj ib txwm; yog tias tsim nyog, nws tuaj yeem muab tso rau ntawm 4℃thaum hmo ntuj kom qeeb qeeb.

Rov qab khov-thaw cycles raug txwv nruj heev: Txhua lub vial yog siv rau ib leeg xwb tom qab muab tshuajthiab rov khov dua -thaw cycles raug txwv nruj heev. Cov tshuaj diluted ua hauj lwm yuav tsum tau npaj thiab siv tam sim ntawd.

Kev daws teeb meem: Tom qab rov kho dua, cov tshuaj yuav tsum yog ntshiab, tsis muaj xim, lossis daj daj. Lub xub ntiag ntawm cov khoom flocculent, turbidity, los yog pom hais tias cov protein yuav muaj aggregated los yog degraded, thiab cov tshuaj yuav tsum tau muab pov tseg.

2. Cia tej yam kev mob:

Unreconstituted lyophilized hmoov:

Ntev - lub sij hawm cia: -80℃(ntev khov). Qhov no yogtus nkaus xwbpom zoo mob rau kev tswj xyuas ntev -lub sij hawm stability.

Txee lub neej: Nyob rau hauv tej yam kev mob ntawm -80℃thiab tiv thaiv los ntawm lub teeb thiab dryness, lub txee lub neej yog feem ntau 12 lub hlis.

nruj me ntsis tiv thaiv los ntawm lub teeb thiab noo noo: Khaws rau hauv lub teeb qub-cov ntaub ntawv pov thawj aluminium foil hnab thiab xyuas kom meej tias qhov ua tau zoo- hauv cov khoom siv desiccant zoo.

Reconstituted daws:

Tam sim ntawd aliquot thiab khov: Tom qab thawing, cov txheej txheem yuav tsum tau nqa tawm ntawm cov dej khov thiab aliquoting yuav tsum ua kom tiavhauv 30 feeb.

Repackage thiab khov: Khaws tam sim ntawm -80 degreetom qab repacking. Nws raug nquahu kom siv tsis pub dhau ib hlis. Cia ntawm -20℃tsis pom zoo.

Kev daws teeb meem: Lub diluted ua hauj lwm tovyuav tsum tsis txhob khaws cia. Nws yuav tsum tau khaws cia rau hauv dej khov thiab siv tsis pub dhau 4 teev ntawm kev npaj.

Kev thauj mus los:

yuav tsum thaujhauv qabdej khov qhuav(-78 degree) tej yam kev mob los xyuas kom meej tias nws tsis thaw thoob plaws hauv tag nrho cov txheej txheem.

Cov ntim khoom thauj yuav tsum suav nrog cov dej khov qhuav txaus, cov thawv ntim khoom, thiab cov cuab yeej ntsuas kub.

3. Kev ruaj ntseg thiab cov qauv ua tiav:

Physicochemical stability:

Nws yog qhov ruaj khov tshaj plaws nyob ze - nruab nrab pH (6.0-7.5) thiab hauv isotonic tsis muaj cov khoom thauj cov protein.

Nws yog rhiab heevmus rau mechanical stress (vortex, oscillation, bubbling), rov khov-thaw cycles, thiab nto adsorption .

Tsis txhob sib cuag nrog cov kua qaub, muaj zog alkalis, detergents, thiab oxidants.

Txhim kho bioactivity:

nruj me ntsis raws li lub hauv paus ntsiab lus ntawm "kev tiv thaiv tus neeg nqa khoom, tuav maj mam, sib sib zog nqus cryopreservation, thiab siv ib zaug -"yog qhov tseem ceeb los xyuas kom meej qhov kev ntseeg tau ntawm kev tshawb fawb tau.

Xav taudab tsi, thov hu raupeb

Cim npe nrov: follistatin 344 1mg/vial muaj zog inhibits myostatin, Tuam Tshoj follistatin 344 1mg/vial muaj zog inhibits myostatin manufacturers, lwm tus neeg, Hoobkas