Follistatin 344 1mg/vial China Peptides Freeze-Powder qhuav

Follistatin 344 1mg/vial core introduction

Pharmaceutical -qib peptide|Follicle-Suppressing Hormone 344 (Ua hauv Suav teb)

Tshuaj npe: Follicle-stimulating hormone 344 fragment

Ib ntus: Tib neeg follicle-stimulating hormone amino acid 1-344

CAS Nr.: 126853-54-1

Specification: 1mg / lub raj mis (lyophilized hmoov)

Daim ntawv noj tshuaj: Lyophilized peptide npaj rau kev txhaj tshuaj

Molecular hnyav: kwv yees li 38.5 kDa

Purity: Ntau dua lossis sib npaug li 95% (RP-HPLC kuaj pom)

Bioactivity: Txheeb xyuas los ntawm activin binding inhibition assay

Cia kub: -20℃rau ntev- lub sijhawm cia, 2-8℃rau lub sijhawm luv

Kev ruaj khov tom qab hloov kho: Txias ntawm 2-8℃thiab siv hauv 24 teev.

Kev thauj mus los: Kev thauj mus los nrog cov ntim dej khov lossis cov dej khov qhuav

Dissolving tswv yim: Cov dej tsis huv rau kev txhaj tshuaj lossis PBS tsis muaj

Core Function|Cov teebmeem tseem ceeb

Follistatin 344 yog afunctional active fragment ntawm follicle-stimulating hormone. Raws li ib tugnatural binding protein ntawm activin thiab myostatin, nws muaj qhov tseem ceeb tshawb fawb tus nqi hauvKev tswj cov nqaij ntshiv, kev tshawb fawb txog kev xeeb tub endocrinology, thiab tshawb nrhiav kev kho mob ntawm cov leeg nqaij.

✅Biologicaltshuab:

Ligand khi: Muaj kev sib raug zoo nrog rau activin A, activin B, thiab myostatin

Teeb liab antagonism: thaiv kev khi ntawm TGF- tsev neeg ligands rau receptors

Kev loj hlob ntawm cov nqaij ntshiv: Relieves inhibition ntawm cov leeg nqaij loj hlob los ntawm myostatin.

Ntau txoj cai: Koom nrog kev tswj hwm ntawm ntau lub cev nrog rau kev tsim tawm, cov metabolism, thiab kev tiv thaiv.

✅Qualityqhov zoo ntawm Suav manufacturing:

Mammalian expression system: HEK293 los yog CHO cell qhia, xyuas kom zoo folding.

Ion pauv purification: multi-qhib chromatography purification kom tshem tawm cov tswv tsev proteins thiab endotoxins.

Endotoxin tswj: <1.0 EU/μg, meeting the standards for injectable protein.

Kev hloov kho glycosylation: Khaws cov qauv glycosylation ntuj thiab khaws cia kev ua haujlwm lom neeg.

✅QualityCov qauv ntsuas:

HPLC purity: RP-HPLC purity Ntau dua lossis sib npaug li 95% (C18 kem, acetonitrile- dej gradient)

SEC-HPLC: Monomer cov ntsiab lus Ntau dua lossis sib npaug li 90%

Loj spectrometry paub meej: MALDI-TOF lees paub qhov hnyav molecular

Western Blot: Kev lees paub ntawm cov tshuaj tiv thaiv tshwj xeeb

Kev tsom xam Peptide mapping: LC-MS/MS raws li kev pom zoo tom qab trypsin digestion

Khoom nta thiab qhov zoo

1. Daim ntawv thov kev tshawb fawb tshawb fawb tus nqi:

Kev tshawb fawb txog nqaij mos biology: Kev tshawb fawb txog kev tswj cov txheej txheem ntawm cov leeg nqaij

Kev tshawb fawb txog kev xeeb tub endocrinology: Kev tsim cov follicle thiab kev tswj hwm cov tshuaj hormones

Kab mob qauv kev tshawb fawb: Muscular dystrophy, Polycystic ovary syndrome

Kev Txhim Kho Tshuaj: Kev Tshawb Fawb Txog Kev Tshawb Fawb Txog Tshuaj rau Kev Kho Mob Cov Kab Mob

2. Technical parameters:

Kev nthuav qhia system: Kev nthuav qhia nyob rau hauv mammalian hlwb, tswj cov haiv neeg conformation.

Lub xeev glycosylation: muaj ntuj N- thiab O-glycosylation hloov kho

Tsis system20 mM phosphate tsis, pH 7.2-7.4

Lyophilization tiv thaiv: Muaj 5% trehalose, 0.01% Tween-80

Isoelectric pointPH 5.8-6.2

3. Qhov zoo ntawm kev tsim khoom hauv Suav teb:

Loj -scale ntau lawm: Mature mammalian cell culture technology

Tus nqi -kev ua tau zoo: Nws muaj tus nqi kom zoo dua piv rau cov khoom tuaj txawv teb chaws.

Kev tswj kom zoo: nruj me ntsis siv cGMP-zoo li cov qauv tsim khoom.

Kev pab txhawb nqa: Ib pab neeg tshaj lij muab kev taw qhia txog kev thov thiab kev sib tham.

Cov kev pabcuam Customization: Peb tuaj yeem muab cov ntawv sib txawv thiab txawv mutant versions.

Daim ntawv qhia kev siv thiab cia

1. Cia tej yam kev mob:

Unopened khov- cov hmoov qhuav: tuaj yeem khaws cia rau 18 lub hlis ntawm -20 degree, thiab rau 24 lub hlis ntawm -80 degree.

Reconstituted daws: tuaj yeem khaws cia ntawm 2-8℃rau 24 teev; zam kom khov.

Repackaging Recommendation: Rau lub sijhawm ntev - khaws cia, nws raug nquahu kom rov ntim thiab khov ntawm -80℃.

Kev tiv thaiv lub teeb: Tsis txhob muaj lub teeb pom kev zoo, tshwj xeeb tshaj yog lub teeb ultraviolet.

Tsis txhob rov khov dua -thaw cycles: Nws raug nquahu kom faib cov khoom rau hauv qhov me me thiab zam ntau tshaj ob qhov khov -thaw cycles.

2. Kev kho dua tshiab:

Kub equilibration: Tom qab tshem tawm ntawm lub freezer, cia kom sib npaug rau chav sov li 15-20 feeb.

Tsis xaiv: Sterile PBS (pH 7.4) lossis physiological saline raug pom zoo.

Reconstitution concentrationPom zoo thawj zaug concentration 0.5-1.0 mg / ml

Cov txheej txheem dissolving: Maj mam ntxiv buffer tov thiab maj mam vortex los yog tig kom yaj.

Kev txiav txim siab concentration: Protein concentration tau txiav txim siab siv txoj kev BCA.

Sterilization los ntawm kev pom: siv 0.22μm qis-protein adsorption lim membrane.

3. Cov lus pom zoo rau kev siv hauv kev sim ntawm tes:

Ua haujlwm concentrationCov lus pom zoo rau kev sim ntawm tes yog 10-1000 ng / ml

Lub sijhawm ua haujlwm: 24-72 teev, hloov kho raws li lub hom phiaj kev sim.

Kev tswj zoo: Nws raug pom zoo kom teeb tsa ib pab pawg kho mob activator A.

Kev ntsuas ntsuas:

Cov leeg nqaij -txog cov noob qhia (MyoD, Myogenin)

SMAD 2/3 phosphorylation theem

Cell proliferation thiab txawv qhov xwm txheej

4. Cov lus pom zoo siv rau hauv kev sim tsiaj:

Dosage rau nas: 1-10 mg/kg lub cev hnyav

Txoj kev tswj hwm: txhaj tshuaj subcutaneous, txhaj tshuaj intraperitoneal, lossis txhaj tshuaj intramuscular

Kev siv ntau zaus: ib zaug ib hnub los yog lwm hnub

Lub sijhawm sim: feem ntau 2-4 lub lis piam

Kev soj ntsuam ntsuas:

Kev hloov ntawm lub cev hnyav thiab cov leeg nqaij

nqaij fiber ntau hla -sectional cheeb tsam

Motor function test

Ntshav biochemical ntsuas

5. Nyob rau hauv vitro binding assay:

ELISA txoj kev:

Coated nrog activator A, kuaj Follistatin khi

Kev sib tw ELISA tshawb pom ntawm myostatin khi

Kev soj ntsuam SPR/BLI:

Txiav txim siab qhov kev sib txuas kinetics ntawm ligands

KD cov nqi feem ntau yog nyob rau hauv pM-nM ntau yam.

Rub -kev sim:

Txheeb xyuas qhov kev cuam tshuam nrog lub hom phiaj protein

Ua ke nrog kev txheeb xyuas qhov tseeb

Tshawb nrhiav thiab siv cov lus qhia

1. Kev tshawb fawb txog cov kab mob ntawm cov leeg:

Tshawb nrhiav kev kho mob rau Duchenne leeg nqaij dystrophy

Pathogenesis ntawm muscular dystrophy

cachexia- cuam tshuam cov nqaij ntshiv

Hnub nyoog -txog sarcopenia

2. Kev tshawb fawb txog tshuaj tua kab mob:

Mechanism ntawm Polycystic Ovary Syndrome

Txoj cai kev loj hlob follicle

Kev mob qog nqaij hlav endocrine

Cov tswv yim tshiab rau kev kho mob ntxiv lawm tshob

3. Kev tshawb nrhiav metabolic:

Cov leeg - rog metabolic cuam tshuam

Kev tswj hwm kev tswj hwm ntawm insulin

Lub zog metabolism sib npaug

Kev rog rog- cuam tshuam txog cov kab mob metabolic

4. Kev Loj Hlob Biology:

Kev loj hlob ntawm embryonic leeg

Kev cai ntawm organogenesis

Cov ntaub so ntswg regeneration mechanism

Stem cell txawv txoj cai

Vim li cas thiaj xaiv Suav - ua Follistatin 344?

Siabkev huv: HPLC purity Ntau dua lossis sib npaug li 95%, kom ntseeg tau qhov tseeb ntawm kev sim.

Siabkev ua si: Qhia nyob rau hauv mammalian hlwb, tswj haiv neeg conformation thiab muaj nuj nqi

nrujkev tswj kom zoo: Ntau-qhov kev tswj xyuas qhov zoo kom ntseeg tau siab batch-rau-batch stability.

Ua kom tiavcov ntaub ntawv: Muab cov ntaub ntawv ua tiav nrog rau COA, MSDS, thiab daim ntawv pov thawj tsom xam.

TechnicalTxhawb nqa: Pab neeg tshaj lij muab kev txhawb nqa rau kev sim tsim thiab kev soj ntsuam cov txiaj ntsig.

Cov ntsiab lus

Cov khoom no yogsiab -purity recombinant human Follistatin 344, tsim sivadvanced mammalian cell qhia thiab protein purification technology nyob rau hauv Suav teb.Nws muablub siab -zoo, muaj zog tshawb nrhiav cov cuab yeejrauCov leeg nqaij biology, kev yug me nyuam endocrinology, thiab kev tshawb fawb txog kev loj hlob biology. Tsim nyogcia tej yam kev mob, txheej txheem kev khiav hauj lwm, thiab tsim nyog sim tsimNws yog ib qho tseem ceeb los xyuas kom meej qhov kev ntseeg tau thiab rov ua dua ntawm cov txiaj ntsig kev tshawb fawb.

Xav taudab tsi, thov hu raupeb

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